中文摘要：

为了有效地保护四川山鹧鸪Arborophila rufipectus这一中国特有珍稀濒危鸟类,本研究采用454 GS FLX对该物种基因组测序,首次利用微卫星搜索软件搜索并初步统计和分析基因组微卫星序列,共搜索到l~6个碱基重复类型的完美型微卫星335 263个。不同类型微卫星中,单碱基重复类型数目最多,为197 913个,约占总数的59.03%,其次是四碱基、三碱基、六碱基、二碱基和五碱基,分别约占微卫星总数的13.59%、8.39%、7.55%、6.49%和4.95%。单碱基微卫星中A重复类型数量最多,两碱基中AC最多,三碱基中AAC,四碱基中AAAC最多,五碱基中AAACA最多,六碱基中AGGGTT最多。A、AGGGTT、AAAC、AC、AAAT、AAC、C、AG、AAAG、AAACA、AAT、AGG、AT、AGC、AAGG、CCG是在所搜索到的四川山鹧鸪微卫星中数量最多的16种重复拷贝类型。本研究深化了对四川山鹧鸪基因组的认识和了解,并为以后开发和筛选大量高质量微卫星标记提供数据支持,也为利用微卫星标记研究更加有效地保护和管理四川山鹧鸪这一珍稀濒危动物奠定了基础。

英文摘要：

The Sichuan hill partridge （Arborophila rufipectus） is a critically endangered species endemic to China. The entire genome of A. rufipectus was sequenced using Roche＇s 454 GS FLX platform. Microsatellites were identified by screen- ing the genome sequences in silico mining and a large number of different kinds of repeat motif types of perfect microsatellite sequences were discovered. A total of 335 263 simple sequence repeats （SSRs） with 1 -6 bp nucleotide motifs were identi- fied. Moreover, A, AC, AAC, AAAC, AAACA, and AGGGTr were the most frequent repeat copy units of the six motif types, respectively. In the different repeat types of the microsatellites, mononucleotide （59.03%） was the most common repeat unit followed by the tetra （ 13. 59% ）, tri （ 8. 39% ）, hexa （ 7. 55% ）, di （ 6. 49% ） and pentanucleotides （4.95%）. In the A. rufipectu~ genome, A, AGGGTY, AAAC, AC, AAAT, AAC, C, AG, AAAG, AAACA, AAT, AGG, AT, AGC, AAGG, and CCG were the 16 predominant repeat types. This study provided significant genome informa- tion of A. rufipectus, which will contribute to further understanding of this species. The resources that we have developed will certainly facilitate the isolation of large numbers of high quality microsatellite markers for genetic diversity studies within species in the future.