采用气相-质谱联用仪(GC-MS)分析了火龙果籽油的脂肪酸组成;利用噻唑蓝(MTT)法,检测了火龙果籽油抑制HepG-2、 HT-29人体癌细胞的增殖,并绘制了火龙果籽油对这些癌细胞生长曲线的影响。结果表明:火龙果籽油中主要含有亚油酸(41.28%)、油酸(26.17%)、棕榈酸(20.76%)等组分,不饱和脂肪酸高达72.10%;MTT法显示火龙果籽油呈剂量(1、3、5 mg/mL)和时间(24、48、72、96 h)依赖方式抑制HepG-2、HT-29等细胞增殖,作用48小时细胞抑制率分别可达到24.76%、38.92%、48.18%和35.47%、46.11%、53.78%,火龙果籽油对这些癌细胞的生长具有显著性、持续性的抑制作用。
The ingredients and contents of fatty acids in Hylocereus Undatus Britt seed oil (HUSO) were determined with GC - MS. The inhibitory effects of HUSO on the proliferation activities, growth curve of hunman cancer cell line HepG- 2 and HT -29 were examined. In addition, the curve about inhibitory effects of HUSO on cancer cell growth was drawed. The results showed that the main fatty acids in HUSO were linolic acid (41.28%), oleie acid (26.17%), and palmitic acid (20.76%). The unsaturated fatty acids were 72.10% of total fatty acids, and the inhibitions of UHSO on the proliferation of HepG-2 and HT-29 were in dose - (1, 3 and 5 mg/mL) and time - (24, 48, 72 and 96 h) dependent manners, and the inhibition rate of HepG -2 and HT -29 cells reached 24.76% , 38.92% and 48.18% , 35.47% , 46.11% and 53.78% after incubation of 48 h respectively. The Hylo-cereus Undatus Britt seed oil had an significant and continued inhibiting effect on the growth of cancer cells.