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火龙果籽油抑制癌细胞增殖作用的研究
  • ISSN号:1003-0174
  • 期刊名称:《中国粮油学报》
  • 时间:0
  • 分类:TS229[轻工技术与工程—粮食、油脂及植物蛋白工程;轻工技术与工程—食品科学与工程]
  • 作者机构:[1]宁波大学食品科学与工程系,宁波315211
  • 相关基金:国家自然科学基金(31301574),宁波市自然科学基金(2013A610159),宁波大学学科项目(xkl1344),宁波大学人才引进项目(ZX2012000031)
中文摘要:

采用气相-质谱联用仪(GC-MS)分析了火龙果籽油的脂肪酸组成;利用噻唑蓝(MTT)法,检测了火龙果籽油抑制HepG-2、 HT-29人体癌细胞的增殖,并绘制了火龙果籽油对这些癌细胞生长曲线的影响。结果表明:火龙果籽油中主要含有亚油酸(41.28%)、油酸(26.17%)、棕榈酸(20.76%)等组分,不饱和脂肪酸高达72.10%;MTT法显示火龙果籽油呈剂量(1、3、5 mg/mL)和时间(24、48、72、96 h)依赖方式抑制HepG-2、HT-29等细胞增殖,作用48小时细胞抑制率分别可达到24.76%、38.92%、48.18%和35.47%、46.11%、53.78%,火龙果籽油对这些癌细胞的生长具有显著性、持续性的抑制作用。

英文摘要:

The ingredients and contents of fatty acids in Hylocereus Undatus Britt seed oil (HUSO) were determined with GC - MS. The inhibitory effects of HUSO on the proliferation activities, growth curve of hunman cancer cell line HepG- 2 and HT -29 were examined. In addition, the curve about inhibitory effects of HUSO on cancer cell growth was drawed. The results showed that the main fatty acids in HUSO were linolic acid (41.28%), oleie acid (26.17%), and palmitic acid (20.76%). The unsaturated fatty acids were 72.10% of total fatty acids, and the inhibitions of UHSO on the proliferation of HepG-2 and HT-29 were in dose - (1, 3 and 5 mg/mL) and time - (24, 48, 72 and 96 h) dependent manners, and the inhibition rate of HepG -2 and HT -29 cells reached 24.76% , 38.92% and 48.18% , 35.47% , 46.11% and 53.78% after incubation of 48 h respectively. The Hylo-cereus Undatus Britt seed oil had an significant and continued inhibiting effect on the growth of cancer cells.

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期刊信息
  • 《中国粮油学报》
  • 中国科技核心期刊
  • 主管单位:中国科学技术协会
  • 主办单位:中国粮油学会
  • 主编:于衍霞
  • 地址:北京市西城区百万庄大街11号
  • 邮编:100037
  • 邮箱:lyxbao@126.com
  • 电话:010-68357510
  • 国际标准刊号:ISSN:1003-0174
  • 国内统一刊号:ISSN:11-2864/TS
  • 邮发代号:80-720
  • 获奖情况:
  • 国内外数据库收录:
  • 被引量:22098