中文摘要：

目的建立测定大鼠血浆中山奈酚的方法。方法以黄芩素为内标,无水乙醚为萃取溶剂,采用HPLC法,Shimm-pack VP-ODS柱为分析柱,乙腈-0.5%冰醋酸(33.3:66.7)为流动相,流速为1.0 ml.min-1,检测波长为370 nm,柱温为40℃。结果山奈酚0.050~5.301μg.ml-1与峰面积比(山奈酚/内标)的线性关系良好,最低定量限为0.020μg.ml-1,4种浓度的平均回收率为96.48%~108.33%,日内RSD≤7.39%,日间RSD≤5.88%,3种浓度的平均萃取回收率为93.67%、98.37%、101.83%。结论所建方法简单、灵敏、准确可靠,可用于山奈酚的药物动力学研究。

英文摘要：

OBJECTIVE To determine the kaempferol in rat plasma.METHODS A liquid-liquid extraction method with absolute ether was used and baicalein was chosen as internal standard.The analytes were separated on a Shimm-pack VP-ODS column at 40℃ and detected at 370 nm.The mobile phase consisted of acetonitrile-0.5% glacial acetic acid(33.3:66.7) with a flow rate of 1.0 ml·min-1.RESULTS The calibration curve of kaempferol in plasma showed a good linearity over the concentration range of 0.050-5.301 μg·ml-1 and the lower...