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中国红、绿茶适制品种(系)遗传多样性与亲缘关系的SSR分析
  • ISSN号:0578-1752
  • 期刊名称:《中国农业科学》
  • 时间:0
  • 分类:S68[农业科学—观赏园艺;农业科学—园艺学]
  • 作者机构:[1]中国农业科学院茶叶研究所国家茶树改良中心,杭州310008, [2]南京农业大学茶叶科学研究所,南京210095
  • 相关基金:基金项目:国家现代农业产业技术体系建设专项资金(nycytx.23)、国家科技基础条件平台建设计划(2005DKA21002)、国家自然科学基金(30972403)、江苏省科技支撑计划(BE2008320~2,BE2009313-1)、苏州市基础条件平台建设计划(SZGD201067)
中文摘要:

【目的】探讨中国红、绿茶适制品种(系)的遗传多样性和亲缘关系,了解遗传背景对于品种适制性差异的影响与作用,为茶树育种研究提供分子生物学方面的参考。【方法】利用自主开发的具有多态性的39对SSR引物对中国157份红、绿茶适制品种(系)进行遗传多样性评价和亲缘关系分析。【结果】引物在供试材料中平均可检测4.90个等位基因,11.28个基因型,平均多态性信息量(PIC)0.53,基因多样性指数(H)0.57,遗传分化系数GST0.19,说明81%的遗传多样性来自品种之间的遗传差异。各省份供试材料的遗传多样性以广东最高,江西最低。按照适制性分组分析,结果表明适制红茶品种(系)的遗传多样性水平最高,红绿茶兼制型品种次之,适制绿茶品种(系)最低。根据育种方式分组分析表明,系统选育法得到的品种(系)其遗传多样性水平高于杂交选育品种(系),高于地方品种(系)。聚类分析显示,供试材料聚为了三大类,大部分品种(系)都按照其地理来源和遗传背景进行聚类,并未按照适制性相互聚类。【结论】中国红、绿茶适制品种(系)具有较高水平的遗传多样性。其中,适制红茶品种(系)的遗传多样性水平最高,红绿茶兼制型品种次之,适制绿茶品种(系)最低。同时,主要依据地理来源和遗传背景进行亲缘关系聚类。

英文摘要:

[Objective] The genetic diversity and relationship of tea cultivars and strains suitable for manufacturing green and black tea were studied in order to learn the genetic effect on the manufacture suitability of tea plant and to provide some molecular information to support the effective tea breeding. [ Method ] The genetic diversity and cluster analysis of 157 cultivars and strains of tea plant were analyzed using 39 pairs of SSR markers. [Result] Totally 191 alleles and 440 genotypes were amplified by 39 SSR markers, with the average of 4.90 and 11.28. The average polymorphism information content (PIC) , genetic diversity (H) and genetic distance were 0.57, 0.53 and 0.65, respectively. Genetic diversity of tested tea cultivars and stains from Guangdong was higher than that from other provinces. Evaluated samples were classified by their manufacture suitability and the genetic diversity of black tea cultivars was higher than that of double-edged cultivars for both black tea and green tea and that of green tea cultivars. The level of genetic diversity among cultivars bred by individual selection was higher than that of cultivars bred by other methods. Almost all the tested cultivars and swains were clustered according to their geographic source and breeding source using UPGMA method rather than manufacture suitability. [Conclusion] The genetic diversity of 157 tested tea cultivars was relatively abundant and the most abundant genetic diversity was identified among cultivars suitable for making black tea. In addition, the samples were clustered based on geographic and breeding source.

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期刊信息
  • 《中国农业科学》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国农业科学院 中国农学会
  • 主编:万建民
  • 地址:北京中关村南大街12号中国农业科学院图书馆楼4101-4103室
  • 邮编:100081
  • 邮箱:zgnykx@caas.cn
  • 电话:010-82109808 82106279
  • 国际标准刊号:ISSN:0578-1752
  • 国内统一刊号:ISSN:11-1328/S
  • 邮发代号:2-138
  • 获奖情况:
  • 中国期刊方阵“双高”期刊,第三届中国出版政府奖提名奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:85620