中文摘要：

白细胞介素10（Interleukin 10,IL-10）是一种抗炎细胞因子,可以抑制机体免疫反应。本研究经过分析大黄鱼基因组数据库发现了IL-10同源基因,并对其cDNA编码区序列和基因组DNA序列进行了克隆分析。大黄鱼IL-10（LycIL-10）基因由5个外显子和4个内含子构成,其序列全长1 869bp,其开放阅读框（ORF）长555bp,编码184个氨基酸,其N端的22个氨基酸残基为预测的信号肽,成熟肽由162个氨基酸残基组成,包含了脊椎动物IL-10标志性保守序列。LycIL-10的氨基酸序列同其他已知物种的IL-10氨基酸序列的一致性为26.49%-77.01%。Real-time PCR分析发现LycIL-10在检测的组织中为组成型表达,在脾脏和肌肉中转录水平相对较高。三联灭活细菌疫苗和聚肌苷酸胞苷酸（poly（I∶C））刺激后,大黄鱼头肾和脾脏中LycIL-10mRNA的转录水平会显著升高,表明LycIL-10可能参与抑制大黄鱼由细菌和病毒引起的炎症反应。

英文摘要：

Interleukin-10（IL-10）is a central anti-inflammatory cytokine that demonstrates immunosuppressive function.In this research,an interleukin-10（IL-10）homologue has been identified by analyzed the genomic data of large yellow croaker（Larimichthys crocea）.Subsequently,the open reading frame（ORF）of cDNA and the genomic DNA sequences have been cloned and analyzed.The genomic DNA of large yellow croaker IL-10（LycIL-10）consisted of 1 869 bp that contains five exons and four introns,sharing the same organization with mammalian IL-10 genes.The open reading frame of LycIL-10 consisted 555bp that give a predicted 184 amino acid IL-10 molecule.It contains a predicted signal peptide of 22 amino acids in the N-terminal and a mature peptide of 162 amino acids,which contained the vertebrate IL-10 family signature.The LycIL-10 exhibits a conserved IL-10 motif signature and shares 26.49%-77.01%amino acid sequence identity with other known IL-10.Real-time PCR analysis showed that LycIL-10 was constitutively expressed in all tissues tested,especially in spleen and muscle.The LycIL-10 mRNA transcription level could significantly increase in the spleen and head kidney of large yellow croaker after trivalent inactivated vaccines or poly（I∶C）stimulated,indicating that LycIL-10 may be involved in suppressing the inflammatory induced by bacterium or virus in the large yellow croaker.