中文摘要：

对植物总RNA的提取和鉴定方法作了改进。以马铃薯叶片和茎为材料，分别用常规苯酚法和改进法提取总RNA，通过甲醛变性胶和非变性胶及紫外光谱分析，表明用改进法提取的总RNA与常规苯酚法无明显差异，RNA完整性良好，可以用普通琼脂糖凝胶代替甲醛变性胶电泳分析RNA的完整性。RT-PCR结果表明，以改进法提取的总RNA可以用于分子克隆和基因表达分析等分子生物学实验。利用改进法提取植物总RNA，能降低成本，节约时间，也避免了使用DEPC和甲醛等试剂。

英文摘要：

An improved method of extracting and identifying total RNAs from potato leaf and stem was put forward. The electrophoretic patterns and absorbance at 260 nm and 280 nm in a UV-Vis spectrophotometer revealed that the total RNAs of potato extracted by this method has good completeness and high purity. Furthermore, the resuh of RT - PCR indicated that the RNAs prepared with this improved method could meet the needs of most molecular biological experiment including gene cloning and expression analysis.