中文摘要：

目的通过比较瘢痕疙瘩及正常皮肤中细胞外信号调节激酶（extracellular signal-regulated kinase,ERK）、应激活化蛋白激酶（c-Jun amino-terminal kinase,JNK）信号通路的表达,探讨其在瘢痕疙瘩形成中的作用。方法取四川大学华西医院烧伤整形科收治的26例患者皮肤组织,其中行瘢痕疙瘩切除患者（实验组）16例,瘢痕疙瘩形成时间8个月～10年;胸部6例,耳垂4例,会阴部2例,肩部3例,腹部1例;均经病理检查确诊为瘢痕疙瘩。10例整形手术患者自愿捐赠的正常皮肤作为对照组,腹部4例,大腿3例,肩部2例,背部1例。取标本采用Envision二步法行免疫组织化学染色,观察磷酸化及非磷酸化JNK和ERK表达情况,并采用Image Pro Plus 4.5图像分析系统测定积分吸光度（IA）值,观察阳性染色强度。结果免疫组织化学染色观察显示,对照组正常皮肤成纤维细胞中未见明显的磷酸化及非磷酸化ERK、JNK阳性表达;而实验组主要在成纤维细胞中表达,阳性颗粒主要位于细胞质和细胞核内。实验组磷酸化ERK及JNK的IA值明显高于对照组（P〈0.05）,非磷酸化ERK及JNK两组间差异无统计学意义（P〉0.05）。结论磷酸化JNK、ERK信号通路蛋白在瘢痕疙瘩中异常高表达,提示该通路可能与瘢痕疙瘩形成密切相关。

英文摘要：

Objective To observe the protein expression of c-Jun amino-terminal kinase（JNK） and extracellular signal-regulated kinase（ERK） in normal skin and keloid and to explore their influences on the formation of kloid.Methods Keloid tissues and normal skin tissues were collected from 16 keloid resection patients（experimental group） and 10 voluntary plastic surgery patients（control group）.In the experimental group,the keloid formation time ranged from 8 months to 10 years;the keloid tissues were collected from the chest in 6 cases,the ear lobe in 4 cases,the perineum in 2 cases,the shoulder in 3 cases,and the abdomen in 1 case;and all keloid tissues were confirmed by pathological examination.In the control group,normal skin tissues were collected from the abdomen in 4 cases,the thighs in 3 cases,the shoulder in 2 cases,and the back in 1 case.Two-step line of Envision immunohistochemical staining was performed to observe the expressions of non-phosphorylated and phosphorylated JNK and ERK;Image Pro Plus 4.5 image analysis system was used to measure the integrated absorbance（IA） and to observe the positive staining strength.Results The immunohistochemical staining showed that no obvious expressions of phosphorylated and non-phosphorylated ERK,JNK were observed in the fibroblasts of the control group,and the expressions of phosphorylated JNK and ERK proteins were significantly higher in the experimental group than in the control group（P 0.05）.There was no significant difference in the expressions of non-phosphorylated JNK and ERK proteins between 2 groups（P 0.05）.Conclusion Activation of ERK and JNK pathways might be involved in formation of keloid.