中文摘要：

目的建立RP—HPLC法同时测定六味地黄渗透泵片中没食子酸、5-羟甲基糠醛、莫诺苷、马钱苷、獐芽菜苷、芍药苷和丹皮酚7种活性成分的含量。方法采用DiamonsilC18色谱柱（250nmm×4．6mm，5μm），以体积分数为0．06％磷酸乙腈溶液（A）和体积分数为0．06％磷酸水溶液（B）为流动相，梯度洗脱，流速为1．0mL·min-1，检测波长为254nm，柱温为35℃。结果没食子酸、5-羟甲基糠醛、莫诺苷、马钱苷、獐芽菜苷、芍药苷和丹皮酚质量浓度分别在3．992～19．96mg·L^-1、8．710-43．55mg·L^-1、5．613～28．06nag·L^-1、8．772—43．86mg·L^-1、0．7217～3．608mg·L^-1、1．643～8．215mg·L“和14．60～73．00mg·L“内与峰面积呈良好的线性关系（r≥0．9997），平均回收率（n=9）分别为96．1％、100．5％、99．1％、101．1％、102．6％、97．8％和102．5％。结论本方法快速简便，具有较好的重复性和稳定性，为六味地黄渗透泵片的质量控制提供参考。

英文摘要：

Objective To develop an RP-HPLC method for simultaneous determination of gallic acid, 5- hydroxylmethyl furfural, morroniside, loganin, sweroside, paeonol and paeoniflorin in Liuwei Dihuang （ tradi- tional Chinese medicine） osmotic pump-controlled release tablets. Methods Isolation of sample was achieved on a Diamonsil C18 column （250 mm ×4. 6 mm,5 i.tm）. The column temperature was maintained at 35 ℃ and detection wavelength was set at 254 nm. Solvent A （water,0. 06% phosphoric acid, V： V） and solvent B （acetonitrile,0. 06% phosphoric acid, V： V） were used for gradient elution. The flow rate was 1.0 mL. min-~. Results The linearity ranges of gallic acid,5-hydroxylmethyl furfural, morroniside, loganin, sweroside, paeonol and paeoniflorin were 3. 992- 19. 96 mg·L^-1, 8. 710- 43. 55 mg·L^-1, 5. 613 - 28. 06 mg ·L^-1 , 8. 772 - 43.86 mg·L^-1,0. 721 7-3.608 mg·L^-1, 1. 643- 8. 215 mg .L-t and 14. 60-73.00 mg ·L^-1 ,respectively （r≥0. 999 7）. The average recoveries （n =9） of them were 96. 1% ,100. 5% ,99. 1% ,101.1% ,102. 6%, 97.8% and 102. 5% ,respectively. Conclusions The method is simple,rapid and accurate. It can be applied for the quality control of Liuwei Dihuang osmotic pump-controlled release tablets.