中文摘要：

目的体外探讨缺血启动新生大鼠未成熟室管膜下区（SVZ）和脑白质内源性修复机制。方法体外培养5日龄内sD新生大鼠的SVZ和脑室周围白质细胞，各分为正常对照组和氧气．葡萄糖剥夺（OGD）组，利用免疫荧光双标技术检测新生祖细胞的增殖分化情况，利用Hoechsd3342／碘化丙啶双染技术和流式细胞术检测新生细胞成活情况。结果OGD组SVZ和脑白质在OGD后24h、48h、72h、7d及14d均出现明显的凋亡和坏死细胞，各时段新生细胞的凋亡率均显著高于同时段对照组（P均〈0．01）。荧光显微镜观察显示，与正常对照组比较，OGD组SVZ和脑白质新生NG2＋祖细胞和04＋少突胶质细胞（OL）前体的细胞数量在OGD后72h内均明显增加（P均〈0．05，0．01），CNPase＋未成熟OL和MBP＋成熟OL在OGD后7d和14d则较同时段正常对照组明显减少（P均〈0．05，0．01）。结论缺血可诱导SVZ和脑白质神经祖细胞激活和明显增殖，并沿OL系分化，但最终仅有极少量新生OL前体能分化为未成熟和成熟OL；同时，缺血导致OGD组新生细胞出现明显凋亡和坏死。

英文摘要：

Objective To explore an endogenous self-repair potentiality for injured cerebral white matter from both of subependymal ventricular zone and white matter cell cultures in neonatal rats with oxygen glucose deprivation （OGD） in vitro. Methods The white matter and subependymal ventricular zone tissues from the neonatal rats within 5 days old were separately used to prepare primary glia-derived cell cultures, and these cell cultures were randomly divi- ded into the control group and the OGD group. The double-label fluorescent immunoanalysis was used to observe the proliferation and differentiation of the glia-derived cells came from both of subependymal ventricular zone and white matter activated by OGD. The Hoechst33342/propidium iodide （PI） staining and the flow cytometry technology were used to assess the apoptotic rates of the newborn cells. Results More apoptotic and necrotic cells appeared in the OGD group than those in the control group both in subependymal ventricular zone and white matter cell cultures in the flow cytometry test and Hoeehst33342/PI staining at 24 h ,48 h ,72 h,7 d and 14 d after OGD （ all P 〈 0.01 ）. Furthermore, fluorescence microscope showed that the number of the NG2 ~ progenitor cells, the 04 ~ oligodendrocyte precursor cells in the OGD group were all significantly more than those in the control group during 72 h after OGD （ all P 〈 0.05, 0.01 ） ,while the number of the immature and mature oligodendroeytes in the OGD group decreased significantly com- pared with those in the control group on 7 d and 14 d after OGD （ all P 〈 0.05,0.01 ）. Conclusions OGD may acti- vate 2 endogenous self-repair pathways from subependymal ventricular zone and white matter in vitro. The activated subependymal ventricular zone and white matter-glial progenitor cells appear to proliferate markedly, and differentiate a- long an oligodendroglial pathway. However, only a few newly generated precursor cells can be differentiated into the im- mature or mature oligodendroeytes and OGD may in