中文摘要：

目的：研究水相中药物分子烙印聚合物的制备方法，并对聚合物的底物识别行为进行研究。方法：以合成的可聚合化的β-环糊精为功能单体，N，N-亚甲基双丙烯酰胺为交联剂，在水相中成功地制备了B受体阻滞药卡维地洛和美托洛尔的分子烙印聚合物，并采用平衡结合试验，在乙腈-水溶剂中考察了该聚合物对模板分子及相似底物的亲和性和选择性。结果：卡维地洛和美托洛尔在水相环境中对原模板具有较高的选择性和识别能力，其吸附行为类似酶与底物的“锁钥”原理，而空白聚合物无此特性。结论：该研究为在水相中合成高识别性能分子烙印聚合物提供了一种切实可行的方法。

英文摘要：

OBJECTIVE： To explore the preparation methods of drug - molecularly imprinted polymer in aqueous phase and the substrate - recognition behaviors of the polymers. METHODS： The β - blocked reagents carvedilol and metoprolol molecularly imprinted polymers were successfully synthesized in aqueous phase by utilizing the polymerisable β- cyclodextrin as functional monomer, N, N- methylene- bisacrylamide as crosslinker, respectively. The affinity and selectivity of imprinted polymers for the templates and its structural analogues were evaluated in acetonitrile - water solvent by equilibrium binding experiments. RESULTS： The carvedilol and metoprolol molecularly imprinted polymers exhibited higher substrate-selectivity and recognition ability toward template, and the adsorption behaviors of the imprinted polymers were same as the ＂key- lock＂ relation between the enzyme and the substrate, however, the blank polymer didn＇t. CONCLUSIONS： The studies provided a feasible way to prepare molecularly imprinted polymers for drugs molecules in aqueous phase with highly recognizing ability.