中文摘要：

目的 观察旋毛虫（Trichinella spiralis）成虫排泄-分泌蛋白（adult-worm excretory-secretory protein, AES）对小鼠脓毒症急性肝损伤的保护作用。 方法 24只雄性BALB/c小鼠采用抽签法随机分为假手术组（Sham组）、盲肠结扎穿孔组（CLP组）、AES治疗组（AES ＋ CLP组）和AES对照组（AES ＋ Sham组）, 每组6只。CLP组和AES ＋ CLP组采用盲肠结扎穿孔术制备脓毒症急性肝损伤动物模型, Sham组和AES ＋ Sham组不结扎、不穿孔盲肠, 其余操作与CLP组相同。术后30 min, Sham组和CLP组腹腔注射PBS, AES ＋ CLP组和AES ＋ Sham组腹腔注射AES（25 μg/只）。术后12 h, 检测小鼠血清中丙氨酸转氨酶（ALT）和天门冬氨酸转氨酶（AST）水平, 观察小鼠肝脏组织病理改变, 蛋白质印迹（Western blotting）测定肝脏组织中Toll样受体4（TLR4）、髓样分化因子88（MyD88）的表达水平, 免疫组化法测定肝细胞核因子-κB（NF-κB）p65的表达水平并分析NF-κB p65核阳性率, ELISA检测小鼠肝脏组织中α肿瘤坏死因子（TNF-α）、白细胞介素-6（IL-6）和IL-1β的水平。 结果 术后12 h, CLP组小鼠血清中ALT和AST水平分别为（269.83±77.60）和（712.00±186.52）IU/L, 明显高于Sham组的（45.00±15.01）和（132.50±36.95）IU/L, 及AES ＋ CLP组的（136.67±30.27）和（419.00±60.86）IU/L（P〈0.05）; AES ＋ Sham组分别为（50.83±13.24）和（134.67±35.78）IU/L, 与Sham组比较差异无统计学意义（P ＞ 0.05）。HE染色显示： CLP组肝索紊乱, 肝细胞水肿, 有炎性细胞浸润; AES ＋ CLP组肝脏结构损伤明显减轻。Western blotting检测显示： CLP组小鼠术后肝脏组织中TLR4/β-actin和MyD88/β-actin相对表达量分别为0.66±0.12和0.69±0.13, 明显高于Sham组的0.31±0.06和0.22±0.04（P〈0.05）; AES ＋ CLP组TLR4/β-actin相对表达量为0.56±0.09, 与CLP组的比较差异无统计学意义（P〉0.05）, 而MyD88/β-actin相对表达量为0.47±0.06,

英文摘要：

Objective To investigate the protective effect of adult-worm excretory-secretory protein （AES） of Trichinella spiralis on sepsis-induced acute liver injury. Methods Twenty-four male BALB/c mice were randomly divided into sham operation group （Sham group）, cecal ligation and puncture group （CLP group）, AES treatment group （AES ＋ CLP group）, and AES control group （AES ＋ Sham group） （n = 6 in each group）. The mice in the CLP group and AES ＋ CLP group received cecal ligation and puncture procedure to induce sepsis-associated acute liver injury. At 30 min after surgery, intraperitoneal injection of PBS or AES （25 μg） was performed. At 12 h after surgery, the serum levels of alanine transaminase （ALT） and aspartate transaminase （AST） were determined and mice were sacrificed to prepare paraffin sections of the liver for HE staining. Protein levels of Toll-like receptor 4 （TLR4） and myeloid differentiation factor 88 （MyD88） in liver were measured by Western blotting. Expression of nuclear factor-κB （NF-κB） p65 in hepatocytes was detected by immunohistochemistry, and the nuclear positive rate was analyzed. The levels of tumor necrosis factor-α （TNF-α）, interleukin-6 （IL-6） and IL-1β in liver were determined with ELISA. Results At 12 h after surgery, the serum levels of ALT and AST in the CLP group [（269.83±77.60） and （712.00±186.52） IU/L, respectively] were both significantly higher than those in the Sham group [（45.00±15.01）, （132.50±36.95） IU/L] and the AES ＋ CLP group [（136.67±30.27）, （419.00±60.86） IU/L] （P〈0.05）, and the serum levels of ALT and AST in the AES ＋ Sham group [（50.83±13.24）, （134.67±35.78） IU/L] had no significantly difference with those in the Sham group. HE staining revealed disordered hepatic cords, hepatocyte swelling, and infiltrations of inflammatory cells in the CLP group, but significantly ameliorated injury in the AES ＋ CLP group. Results of Western blotting showed that the rel