以红松为材料,研究了干旱及复水对红松针叶和树皮绿色组织的黄酮、单宁和原花青素这三种次生代谢产物的含量及其提取物对DPPH清除能力的影响。结果表明:干旱胁迫下,红松树皮绿色组织黄酮、单宁和原花青素含量均显著下降,复水后黄酮和单宁含量持续降低,三种含量均未恢复到对照水平;针叶黄酮和原花青素含量明显降低,单宁含量明显升高,复水后均未恢复到对照水平。干旱胁迫使红松针叶和树皮绿色组织IC50值均明显升高,相对应提取物的DPPH清除能力均明显下降,复水后针叶和树皮绿色组织提取物对DPPH清除能力恢复至对照水平。本研究结果表明干旱胁迫对红松针叶和树皮绿色组织次生代谢产物及抗氧化活性均产生一定影响,但针叶和树皮绿色组织的反应存在一定差异。
The Korean pine were tested to study the effects of drought stress and rehydration on flavonoids content, tannin content, procyanidin content and DPPH free radical scavenging activity of needles and bark chlorenchyma. Flavonoids, tanninand procyanidin content of bark chlorenchyma decreased significantly under drought stress, flavonoids and tannin content continue to decrease after rehydration, and three kinds of content were not returned to control levels. Flavonoids and procyanidin content of needles decreased and tannin content significantly increased, after rehydration they were not returned to control levels. IC50 values of needles and bark chlorenchyma significantly increased under drought stress, and DPPH free radical scavenging activity of extract was significantly decreased, after rehydration DPPH free radical scavenging activity of needle and bark chlorenchyma were returned to control levels. Secondary metabolites and antioxidant activity of needles and bark chlorenchyma were significantly affected by drought stress, but there were some different responses to drought stress between needles and bark chlorenchyma.