中文摘要：

为了降低黄芩组培苗的玻璃化率并提高其生根率，本研究以黄芩无菌苗茎段诱导的不定芽为实验材料，分别研究了6-苄基嘌呤（6-BA）、蔗糖、琼脂及多效唑（PP333）等组培条件对黄芩不定芽玻璃化的影响以及吲哚丁酸（IBA）对再生苗生根的影响。研究结果表明：低浓度的6-BA有利于降低不定芽的玻璃化率，当培养基中添加0．2mg·L^-1 6-BA时，不定芽的玻璃化率最低且增殖系数比对照增加1倍。随着培养基中蔗糖浓度的升高，不定芽的玻璃化率显著降低，其增殖系数也有所降低。培养基中蔗糖浓度为25g·L^-1时，黄芩不定芽长势最好且玻璃化率为零，增殖系数也最高。培养基中添加7．5g·L^-1的琼脂，不定芽的玻璃化率较低，增殖系数也较高。培养基中添加多效唑（PP333）有利于缓解黄芩再生不定芽玻璃化状态，随着PP333的浓度增加，玻璃化率也逐渐降低。0．2mg·L^-1PP333对黄芩不定芽的壮苗起到了很好的作用，再生苗明显变得粗壮。0．1mg·L^-1的IBA最有利于黄芩再生苗的生根，生根率为100％，平均生根数为7，移栽成活率达95％以上。

英文摘要：

For reducing hyperhydric rate and improving rooting percentage of in vitro regenerated shoots from Sct~tellariae baicalensis Georgi, with the regenerated shoots induced from aseptic seedling stems, we studied the effect of N6-Benzyladenine（6-BA）, sucrose, agar and paclobotrazol （PP333） on hyperhydricity of regenerated shoots in S. baicalensis and the effects of IBA on rooting of regenerated shoots. The hyperhydrie rate significantly decreased with lower 6-BA concentration on the medium. The lowest hyperhydric rate and the highest multiplication rate were on the medium containing 0. 2 mg·L^-1 6-BA. With the sucrose concentration increasing, the hyperhydric rate of S. baicalensis adventitious shoots was reduced, while their multiplication coefficient was also reduced. The best multiplication coefficient in vitro shoots without hyperhydricity were found on the medium containing 25 g·L^-1 sucrose. On the medium containing 7.5 g·L^-1 agar, the hyperhydric rate of S. baicalensis adventitious shoots was the lowest with higher proliferation coefficient. The medium containing PP333 positively affected the hyperhydric occurrence of regenerated shoots. With the increase of PP333 concentration on the medium, the hyperhydric rate was decreased gradually and in vitro shoots were stronger and healthier. The optimal rooting was got from the medium containing 0.1 mg·L^-1 IBA, with the rooting rate of 100% and seven roots per shoot. The survival rate of rooting plantlets was over 95% after transferred to soil.