2009年4月、7月和10月分别在内蒙古农牧交错区元上都、黑城子和大河乡样地采集沙蒿(Artemisia sphaerocephala)根围土壤样品,利用形态学和微形态学特征分离鉴定沙蒿根围AM真菌种类,研究了AM真菌物种多样性.共分离鉴定出AM真菌3属20种,其中盾巨孢囊霉属(Scutellospora)2种,无梗囊霉属(Acaulospora)4种,球囊霉属(Glomus)14种.3属AM真菌在3个样地均有分布.3个样地共同优势种是黑球囊霉(G.melanosporum)和网状球囊霉(G.reticulatum).AM真菌物种丰度、香浓-威纳指数和均匀度在时间上表现为4月〈7月〈10月,空间上表现为元上都〈黑城子〈大河乡.扫描电镜下不同属种孢子表面纹饰特征差异明显,可作为AM真菌分类的参考依据.
To study AM fungal species diversity and its spatio-temporal distribution,soil samples were collected in the rhizosphere of Artemisia sphaerocephala from three sites of Inner Mongolia in April,July and October 2009,and morphological and micro-morphological characteristics separation-identification methods were used.The results showed that 20 AM fungal species in three genera were isolated and identified from the rhizosphere of A.sphaerocephala in Inner Mongolia,2 species belong to Scutellospora,4 to Acaulospora and 14 to Glomus.G.melanosporum and G.reticulatum were the dominant species in three sites.AM fungal species richness,Shannon-Wiener index and evenness displayed AprilJulyOctober in time,and YuanshangduHeichengziDahexiang on space.SEM showed that fungal spore morphology was different among species,especially in surface ornamentation,that could be used as a reference index for AM fungal identification.