中文摘要：

【目的】柞蚕核型多角体病毒（Antheraea pernyi nucleopolyhedroviruses,AnpeN PV）能引起柞蚕脓病大面积暴发。尽管之前已完成了两株核型多角体病毒的基因组测序,但基于比较基因组的方法调查AnpeN PV间的遗传变异研究则相对较少。【方法】为了研究AnpeN PV不同地理株系间的遗传多样性,我们测序了1株从中国河南省分离的柞蚕NPV（AnpeN PV-H）基因组,并和之前从辽宁省分离的2株柞蚕NPV（AnpeN PV-L和AnpeN PV-Z）进行了比较基因组学分析。【结果】AnpeN PV-H的全基因组大小为125 605 bp,总共预测了146个开放阅读框（ORF）,包括95个功能注释蛋白和51个假定蛋白。我们发现这3株AnpeN PV间的基因组成非常相似。在这3个AnpeN PV株系间,有6个开放阅读框存在碱基变异而导致基因的变短。并鉴定超过200个单核苷酸多态性（single nucleotide variations,SNVs）,其中85%位于蛋白编码区。同时,odv-e56,p94-like和egt 3个基因的非同义突变较高,表明这3个蛋白质编码基因可能经历了正向选择或纯化选择。我们发现在这3株AnpeN PV间一些基因的氨基酸发生变异,例如编码膜蛋白的基因、抑制凋亡和蜕皮的基因及与DNA复制相关的DNA聚合酶和解旋酶等基因。最后,展示了3株AnpeN PV间遗传重组的证据。【结论】本研究揭示了AnpeN PV种内水平的变异和株系内基因组的动态结构。

英文摘要：

【Aim】Antheraea pernyi nucleopolyhedrovirus（ Anpe NPV） produces epizootic outbreaks in populations of the oak silkworm A. pernyi. We conducted a comparative analysis among three Anpe NPV isolates to gain insight into the host / parasite interactions of this system at the molecular level. 【Methods】We sequenced the genome of an Anpe NPV（ Anpe NPV-H） isolated from Henan province,China and performed a comparative analysis with two previously sequenced isolates（ Anpe NPV-L and Anpe NPV-Z）from Liaoning province. 【Results】The Anpe NPV-H strain is 125 605 bp in length and contains 146 open reading frames of which 95 are identified as functional proteins and 51 are annotated as hypothetical proteins. The genetic arrangement（ synteny） of the three strains is identical while six ORFs are truncated by stop codons in one or two of the isolates. There are more than 200 single nucleotide variations（ SNVs）among the three Anpe NPV strains,85% of which are found in coding regions. Fifty genes show amino acid changes,three of which（ odv-e56,p94-like,and egt） have higher evolutionary rates of change（ d N/d S 1）,suggesting positive or purifying selection. Significant amino acid differences（ changes in charge or hydrophobicity among isolates） are seen in envelope proteins,DNA polymerase,helicase and proteins involved in apoptosis and ecdysis. Finally,we show evidence of genetic recombination between Anpe NPV strains. 【Conclusion】This study reveals the level of intra-species variation among the strains examined and lends insight into host / virus interactions based on the dynamics of the A. pernyi nuclear polyhedrosis virus（ Anpe NPV） genome.