中文摘要：

为了研究水杨酸（SA）对高温强光胁迫下小麦叶片类囊体膜D1蛋白磷酸化和PSⅡ功能的影响，用0．5mmol·L^-1SA溶液预处理灌浆期小麦叶片，以水预处理为对照，然后将预处理植株进行高温强光（35℃，1600μmol·m^-2·s^-1）处理，测定胁迫处理过程中小麦旗叶光合电子传递速率、净光合速率、叶绿素荧光参数及D1蛋白的变化．结果表明：SA预处理有效抑制了高温强光下D1蛋白的净降解，保持了较高的D1蛋白磷酸化水平、全链电子传递速率和PSⅡ电子传递速率，维持了较高的PSⅡ原初光化学效率（Fv／Fm）、实际光化学效率（ΦPSⅡ）、光化学淬灭系数（qp）和净光合速率（Pn）．表明外源SA通过调节小麦叶绿体D1蛋白的周转，减轻了高温强光胁迫对叶片光合机构的损伤，有利于PSⅡ的正常运转．

英文摘要：

To study the effects of salicylic acid （SA） on the D1 protein phosphorylation and PS Ⅱ performance in wheat （Tritivum aestivum L. ） leaf chloroplasts under high temperature and high light, the wheat leaves at grain-filling stage were sprayed with SA solution （0.5 mmol · L^-1 ） or water （as control） , and then subjected to 35 ℃ and 1 600 μmol · m^-2 · s^-1 for various hours. The changes in electron transport rate （ETR） , net photosynthetic rate, chlorophyll fluorescence parameters, and relative amount of phosphorylated and nonphosphorylated D1 protein in thylakoid mem- brane were compared. The results showed that foliar spraying SA effectively inhibited the degradation of D1 protein under high temperature and high light stress, and maintained the D1 protein phosphorylation, ETR of whole chain and PS Ⅱ , Fv/Fm （ the maximal photochemical efficiency of PS Ⅱ）, ΦPsⅡ （the actual photochemical efficiency of PS Ⅱ）, qp（ the photochemical quenching coefficient）, and P. （net photosynthetic rate） at a higher level, indicating that exogenous SA could mitigate the damage effect of high temperature and high light on wheat leaf photosynthetic apparatus and benefit PS Ⅱ performance via regulating the turnover of D1 protein in chloroplasts.