[Objective] The study aimed to prove effects of NO on oxidative damage and photosynthetic apparatus at filling stage of wheat leaves under high temperature and irradiance stress.[Method] Yunong 949 was taken as experimental material to study the effects of sodium nitropprusside (SNP,an exogenous nitric oxide donor) at 0.1 mmol/L on protective enzyme activities, oxidative damage and fluorescence.[Result] The treatment with SNP (at 0.1 mmol/L) significantly increased the activity of SOD and APX, proline content, decreased the MDA content and relative electrical conductivity, Kept the higher Fv/Fm and lower Fo.[Conclusion] The adaptability of wheat with SNP treatment at 0.1 mmol/L was improved under high temperature and irradiance stress.