中文摘要：

目的筛选出稳定过表达p50基因的细胞克隆株ECV-p50,为研究dynei-n-dynactin复合体在登革病毒逆行性运输中的作用奠定基础。方法将构建的pRe-p50质粒稳定转染至ECV304细胞,通过间接免疫荧光染色和免疫印迹实验进行鉴定。结果经间接免疫荧光染色和免疫印迹实验验证,稳定过表达p50基因的细胞克隆株命名为ECV-p50。结论成功建立了稳定过表达p50基因的细胞克隆株ECV-p50,为后续研究积累了有价值的实验资料。

英文摘要：

Objective To construct stable pS0 gene overexpression cells for studying the role of dynein-dynactin in the retrograde transport of Dengue virus. Methods ECV304 ceils were stable transfected by pRe-p50. Then the stable pS0 gene overexpression cells were con- firmed by western blot and indirect immunofluorescence assay. Results The stable p50 gene overexpression cells were selected and identified,named ECV-p50. Conclusion The stable p50 gene overexpression ceils have been constructed successful, which lays a base for further study on roles of p50 in virus infection.