中文摘要：

【目的】观察穿山龙水溶性总皂苷对体外培养类风湿关节炎患者成纤维样滑膜细胞核因子KB（NF-KB）p65及其抑制因子IKB-α蛋白水平和mRNA含量的影响。【方法】培养类风湿患者成纤维样滑膜细胞，分为穿山龙水溶性总皂苷低、中、高剂量组（中药低、中、高剂量组，10、20、30mg／L），模型组，同时设立空白对照组；经药物干预后．采用Westemblot法测定NF-KB和IKB-α蛋白水平，采用半定量逆转录一聚合酶链反应（RT-PCR）方法检测成纤维细胞中NF-KB和IKB-αmRNA的表达。【结果】经穿山龙干预后的关节炎患者成纤维样滑膜细胞的NF-KB活化受到显著抑制，蛋白及基因表达水平较模型组显著下降（P〈O．05），IKB-α蛋白及基因表达显著提升（P〈O．05）。【结论】穿山龙水溶性总皂苷可以抑制类风湿关节炎患者成纤维样滑膜细胞的核转录因平的表达．这可能是穿山方．治疗娄风湿姜节类的机理之一

英文摘要：

Objective To observe the effect of water-soluble total saponin of Rhizoma Dioscoreae Nipponicae （RDN） on protein levels and mRNA contents of nuclear factor-kappa B （NF-KB） p65 and its inhibition factor IKB-α in fibroblast-like synoviocytes of rheumatoid arthritis patients in vitro. Methods Fibroblast-like synoviocytes of rheumatoid arthritis patients were cultured, and then were divided into four groups, namely 10, 20 and 30 mg/L water-soluble total saponin of RDN groups, model group and blank control group. The levels of NF-KB and IKB-α protein were assessed by Western blotting method and their mRNA expression levels were measured by semi-quantitative RT-PCR method. Results The activation of NF-KB in fibroblast-like synoviocytes was significantly inhibited in the three RDN groups , and the protein and gene levels of NF-KB were obviously decreased （P〈0.05, compared with those in the model group） , while the protein and gene levels of lKB-α were increased （P〈0.05）. Conclusion Water-soluble total saponin of RDN can effectively inhibit the expression of nuclear transcription factor in fibroblast-like synoviocytes of rheumatoid arthritis patients, which may be one of the therapeutic mechanisms of RDN for rheumatoid arthritis.