中文摘要：

目的探讨穿山龙总皂苷对Ⅱ型胶原诱导性关节炎（CIA）大鼠血清及滑膜组织肿瘤坏死因子α（TNF-α）的影响，探讨其治疗类风湿关节炎（RA）的免疫调节机制。方法选取48只清洁级Wistar大鼠，随机分成4组：正常对照组（A组）、模型组（B组）、雷公藤多苷片组（C组）和穿山龙总皂苷组（D组），每组12只。B、C、D组大鼠均建立CIA大鼠模型，待模型建立成功后，C、D组大鼠分别给予相应的药物灌胃；A、B组大鼠给予等体积0．9％氯化钠注射液灌胃；均连续给药21d，然后处死大鼠取材。应用双抗体夹心酶联免疫吸附（ELISA）法检测4组大鼠血清TNF-α水平，同时应用逆转录联合酶链式反应（RT—PCR）方法测定4组大鼠滑膜组织TNF-α mRNA表达量。结果B组大鼠血清TNF-α水平及滑膜组织TNF-α mRNA表达量均高于A组，差异有统计学意义（P〈0．05）；而C、D组大鼠血清TNF-α及滑膜组织TNF-α mRNA表达量均低于B组，差异有统计学意义（P〈0．05）；但C、D组大鼠上述指标比较，差异无统计学意义（P〉0．05）。结论穿山龙总皂苷可能通过调节细胞因子TNF-α的水平来发挥其对RA有益的免疫调节作用。

英文摘要：

Objective To study the influence of total saponin from Rhizoma Dioscoreae Nipponicae （TSRDN） on TNF - α in serum and synovial tissues in collagen - induced arthritis rats in order to investigate its immunal regulation in treating rheumatoid arthritis （RA） . Methods 48 clean Wistar rats were randomly divided into four groups： control group （group A）, model group （group B）, tripterygium group （group C） and TSRDN group （group D ） with each group 12 rats. CIA rat models were established in group B, C and D before the rats in group C and D were given intragastrie administration of related medi- cines. Rats in group A and B were given intragastric administration of 0. 9% Sodium chloride injection with the same volume. All the rats were treated for 21 days before then were killed. ELISA method was used to detect TNF - α in serum, and RT - PCR method was used to detect the expression of TNF - α mRNA in synovial tissues. Results The TNF -α expression in serum and TNF - α mRNA expression in synovial tissues in group B were both significantly higher than those in group A （ P 〈 0.05 ） . The TNF -α expression in serum and TNF -α mRNA expression in synovial tissues in group C and D were both significantly lower than those in group B （ P 〈 0.05 ） . But the difference between group C and group D showed no statistically significant difference （ P 〉 0.05 ） . Conclusion Regulating the levels of eytokines TNF - α may be the possible mechanism of TSRDN treating RA.