为了得到较高的丁醇浓度,以汽爆玉米秸秆为原料,对6种糖化工艺路线进行对比研究.结果表明:首先加入纤维素酶量的1/3,反应24 h后,再加入1/3纤维素酶,剩下的纤维素酶再隔12 h加入,共糖化48 h是一条较佳的糖化工艺路线.在此基础上,通过单因素试验和正交试验的考察,得到较优的丁醇发酵工艺条件.采用优化的工艺条件进行发酵,丁醇浓度达到了9.42 g/L.
Several processes of saccharification based on steam-exploded corn stalk were compared in this pa-per, and the best process of saccharification is: adding 1/3 cellulase firstly, adding 1/3 cellulase after 24 h, then adding the rest of the cellulase after 12 h, and saccharification 48 h in all. On this basis, the better con-ditions of fermentative process were investigated by single-factor experiment and orthogonal test. Under the op- timum conditions, the concentration of butanol can reach 9.42 g/L.