中文摘要：

目的观察AMLIB、AML1／ETO对TSC基因、TSC1和TSC2启动子的转录调节作用，探讨其在白血病发生中的作用。方法构建TSC基因启动子／增强子区包含AML1基因结合位点的荧光素酶报告基因质粒，与AMLIB、AML1／ETO表达质粒共转染非洲绿猴肾细胞系CV-1细胞，测定荧光素酶的活性，分析其对TSC基因启动子转录活性的影响。结果在CV-1细胞中，AML1B对TSC1基因启动子的转录没有明显的作用，而对于TSC2基因启动子的转录活性具有明显的促进作用。在pCMV5-AMLIB的剂量为75ng时，TSC2启动子的转录活性上升为对照组的8．55倍，且这种作用具有一定的剂量依赖性；相反，AML1／ETO对TSC1基因启动子转录活性具有明显的促进作用而对于TSC2基因启动子的转录活性没有明显的作用，但是可以抑制AML1B对TSC2基因启动子的反义启动作用。结论AML1B和AML1／ETO能够调控TSC基因的转录。

英文摘要：

Objective To investigate the effects of AMLIB and AML1/ETO fusion gene on the transcription activity of TSCI and TSC2 promotor and to explore its role in leukemogenesis. Methods The luciferase reporter plasmids of TSCs gene promoter containing a AML1 binding site were constructed, and cotransfected into CV-1 cells with AML1B or AML1/ETO expression plasmids separately. The transactivity of TSCs gene promoter was assayed by luminometer. Results AML1B exhibited a transactivity to TSC2 gene promoter in a dosage-dependant manner, but showed no significant transactivity to TSCI ＇ s. The transactivity to TSC2 gene promoter showed 8.55 fold increase as companed with control group at 75 ng of pCMVS-AML/B. AML1/ETO showed a significant transactivity to TSC1, but no transactivity to TSC2＇ s. However, AML1/ ETO antagonized the effect of AML1B to TSC2 gene promoter. Conclusion AML1B and AML1/ETO can regulate the transcription of TSC genes.