有颜色的棉花自然地有 pigmented 纤维。在棉花纤维的色素沉着的机制很好没被记录。这个实验被进行学习呼吸的链禁止者的效果,即,毒鱼藤素和 thiourea,在在有颜色的棉花的色素沉着和纤维开发上。在 1 d 以后开花期以后,卵巢被收获,发展中的小卵在为 30 d 包含毒鱼藤素和 thiourea 的不同集中的液体媒介上是有教养的。结果证明两个呼吸禁止者在小卵文化条件下面减少了纤维长度和小卵开发,并且毒鱼藤素的抑制效率比 thiourea 的高得多。毒鱼藤素和 thiourea 也在纤维颜料(颜色) 上显示出重要效果在有颜色的棉花的开发。在绿棉花纤维,当 thiourea 根本禁止了纤维色素沉着时,毒鱼藤素在 200 mol/L 由 7 d 推进了纤维颜料开发处理层次(400, 600, 800, 1 000,和 2 000 mol/L ) 。然而,两个呼吸禁止者没在棕色的棉花纤维的色素沉着上有重要效果。细胞色素 c oxidase (艇长) 和多酚 oxidase (PPO ) 的活动与增加两个呼吸禁止者的层次显著地减少了。两个呼吸禁止者在译解有重要角色,这被建议在有颜色的棉花的色素沉着和纤维开发的机制。
Colored cotton has naturally pigmented fibers.The mechanism of pigmentation in cotton fiber is not well documented.This experiment was conducted to study the effects of respiratory chain inhibitors,i.e.,rotenone and thiourea,on pigmentation and fiber development in colored cotton.After 1 d post-anthesis,ovaries were harvested and developing ovules were cultured on the liquid medium containing different concentrations of rotenone and thiourea for 30 d.The results demonstrate that both respiratory inhibitors reduced fiber length and ovule development under ovule culture conditions,and the inhibition efficiency of rotenone was much higher than that of thiourea.Rotenone and thiourea also showed significant effects on fiber pigment (color) development in colored cotton.In green cotton fiber,rotenone advanced fiber pigment development by 7 d at 200 μmol/L,while thiourea inhibited fiber pigmentation at all treatment levels (400,600,800,1000,and 2000 μmol/L).Both respiratory inhibitors,however,had no significant effects on pigmentation of brown cotton fibers.The activities of cytochrome c oxidase (COX) and polyphenol oxidase (PPO) decreased significantly with increasing levels of both respiratory inhibitors.It is suggested that both respiratory inhibitors have important roles in deciphering the mechanism of pigmentation and fiber development in colored cotton.