中文摘要：

目的：了解职业苯接触及慢性苯中毒工人外周血中T细胞特异性转录因子T-bet和GATA-3mRNA表达情况。方法：利用SYBR Green I实时荧光定量PCR分别检测20例正常人、25例职业苯接触工人和27例慢性苯中毒工人外周血单个核细胞T-bet和GATA-3 mRNA表达情况。结果：在职业接触苯工人组及慢性苯中毒工人组,多数样本表现为GATA-3表达上升和T-bet表达下降,而少数病人则表现为相反的模式。GATA-3在正常人的表达水平为0.39±0.22,与正常组对照比较职业接触苯工人组中有19例GATA-3表达水平呈上升趋势（0.57±0.54）,慢性苯中毒工人组中则有20例GATA-3表达水平呈上升趋势（0.52±0.50）。此外,在职业接触苯工人组中发现6例GATA-3表达水平显著下降（0.15±0.12,P〈0.05）,同样在慢性苯中毒工人组中也有7例GATA-3表达水平显著下降（0.07±0.06,P〈0.05）。T-bet在正常人的表达水平为2.15±1.45,与正常组对照比较职业接触苯工人组中有19例T-bet表达水平呈下降趋势（1.91±1.49）,慢性苯中毒工人组中T-bet表达水平均呈下降趋势（1.52±0.56）。此外,在职业接触苯工人组中也可发现6例T-bet表达水平显著上升（3.19±2.10,P〈0.05）。结论：职业苯接触及慢性苯中毒影响工人外周血中T细胞特异性转录因子T-bet和GATA-3mRNA表达水平。

英文摘要：

AIM： To investigate the mRNA expression level of T cell-specific transcription factors T-bet and GATA-3 in peripheral blood of benzene-exposed and poisoning workers. METHODS： The mRNA expression of T-bet and GATA-3 in peripheral blood mononuclear cells（PBMCs） from 20 normal individuals,25 benzene-exposed workers and 27 benzene poisoning workers was quantitatively detected by real-time PCR using SYBR green I.RESULTS： Mostly,the mRNA expression of GATA-3 showed an increased trend while T-bet showed a decrease in PBMCs in benzene-exposed group and poisoning group.However,a few cases still showed an opposite pattern.The mRNA expression of GATA-3 in normal group was 0.39±0.22,in 19 cases of benzene exposed workers was 0.57±0.54,and in 20 cases of benzene poisoning workers was 0.52±0.50.GATA-3 expression levels of benzene-exposed workers（6 cases） and of poisoning workers（7 cases） were 0.15±0.12 and 0.07±0.06,respectively,significantly lower than that in normal group（P0.05）.T-bet expression levels were 1.91±1.49 in benzene-exposed group（19 cases） and 3.19±2.10 in benzene-exposed group（6 cases）.The latter was significantly higher than that in normal group（2.15±1.45,P0.05）.The expression level of T-bet in benzene poisoning group was 1.52±0.56.CONCLUSION： Exposure to benzene affects T cell immune functions.The expression of T-bet and GATA-3 in PBMCs of benzene-exposed and poisoning workers contributes to evaluating the differentiation changes of Th1 and Th2.