中文摘要：

目的：了解转录因子T-bet和Gata-3基因在脐带血中的表达情况。方法：采用实时定量RT-PCR检测21例脐带血单个核细胞（CBMC）中转录因子T-bet和Gata-3基因的表达水平,21例健康成人外周血单个核细胞（PBMC）作为对照。结果：T-bet基因和Gata-3基因在CBMC和PBMC中均存在一定表达,Gata-3基因在CBMC中的表达水平（0.43±0.35）%与PBMC（0.42±0.27）%相比无明显差异（P=0.925）,而T-bet基因在CBMC中的表达水平（0.47±0.48）%则明显低于健康成人组（2.12±1.48）%（P=0.000）。结论：CBMC中T-bet基因表达低下,可能与脐带血移植后GVHD发生率低下以及发生程度较轻有关。

英文摘要：

Aim：In order to evaluate the expression level of transcription factors T-bet and Gata-3 mRNA in umbilical cord blood from humans. Methods： Real-time PCR was used for detecting T-bet and Gata-3 genes expression level in 21 cases of mononuclear cells from umbilical cord blood（CBMC）.21 cases of peripheral blood mononuclear cells（PBMC） of healthy adults served as controls.Results： T-bet and Gata-3 genes were expressed in all cases from both CBMC and PBMC.The expression level of Gata-3 gene（0.43%±0.35%） in CBMC was not significant difference in comparison with PBMC（0.42%±0.27%）（P=0.925）.Compared with PBMC（2.12%±1.48%）,the expression level of T-bet gene from CBMC was obviously down-regulated（P=0.000）.Conclusion：The down-regulated expression of T-bet gene might relate to the reduced incidence and severity of GvHD after allogeneic transplantation of umbilical cord blood.