中文摘要：

以金钗石斛（Dendrobium nobile）、流苏石斛（D.fimbriatum）和报春石斛（D.primulinum）为研究对象，研究了其在激素浓度配比下种子萌发情况，并对流苏石斛幼苗进行离体保存。结果表明：25℃时，Knudson C（KC）＋BA2mg／L＋N从0．2mg／L最利于金钗和流苏石斛原球茎诱导和幼苗分化；KC＋NAA0．5mg／L时最适报春石斛原球茎诱导和幼苗分化。幼苗保存在15℃，每天12h光照（20001x），添加3％甘露醇（mannitol）的KC培养基中，10个月后存活率为96％。

英文摘要：

Effect of different hormones combinations on the seed non-symbiotic germination of D. nobile, D. fimbriatum and D. primulinum also the in vitro conservation of D. fimbriatum seedings were studied in this paper. The results showed that KC＋ BA2mg/L ＋NAA0.2mg/Lat 25℃ was the best for D. nobile and D.fimbriatum protcorm growth and organogenesis; KC＋ NAA0.5mg/L at 25 ℃ was suitable for D. primulinum. Addition of mannitol （ 3.0%w/v ） to the medium and incubation at low temperature （ 15 ℃ ） resulted in higher survival rate （ 96% ） after 10 months of continuous storage.