中文摘要：

为探究温度胁迫对凡纳滨对虾肝胰腺氧代谢和能量代谢的影响,本研究采用生物化学方法分析了低温及高温胁迫对凡纳滨对虾肝胰腺中超氧阴离子（O2^-·）产生,超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活力及谷胱甘肽（GSH）含量,一氧化氮合酶（NOS）活力及其催化的一氧化氮（NO）生成量,以及对三磷酸腺苷（ATP）含量的影响。结果表明,15℃低温及31与35℃的高温胁迫均会导致O2^-·显著增加,在15-31℃内,SOD活力与O2^-·含量变化趋势相似,低温胁迫导致CAT活力升高更为显著,低温与高温胁迫后,GSH含量均显著增加;高温胁迫会引起NOS活力及其催化的NO生成量显著增加;低温与高温胁迫均会引起ATP含量显著增加,但是随着温度回复至对照水平,ATP含量也回复至对照水平。结论：低温和高温胁迫均会引起凡纳滨对虾肝胰腺组织中氧代谢失衡,SOD、CAT及GSH在机体应对低温及高温胁迫导致的氧化损伤中可能扮演不同角色,NO介导的信号传导可能在应对高温胁迫中起着重要作用;低温及高温均会导致ATP含量增加,提示对虾在应对温度胁迫的过程中可能需要更多能量。推测环境温度过低或者过高时,可能由于超出凡纳滨对虾氧-温度忍受极限（OCLTT）而导致氧代谢失衡,并对凡纳滨对虾的能量代谢及其他生理活动产生重要影响。

英文摘要：

The aim of this paper is to investigate the impact of temperature stress on the oxygen and energy metabolism in hepotopancreas of shrimp Litopenaeus vannamei. In the present study,the impact of low and high temperature on the superoxide anion（ O2^-·） production,superoxide dismutase（ SOD） activity,catalase（ CAT） activity and glutathione（ GSH） concentration,and the nitric oxide synthase（ NOS） activity and the synthesis of nitric oxide（ NO） was detected by their corresponding biochemical methods. In addition,the concentration of adenosine triphosphate（ ATP） was also examined. The results show ed that O2^-· production could be induced significantly after cold stress at 15 ℃ and heat stress at 31 and 35 ℃. SOD activity was show ed a similar changing profile with the concentration of O2^-· after thermal stress between 15 and 31 ℃.Compared with heat stress,CAT activity was enhanced more significantly after cold stress. How ever,GSH concentration increased significantly after high temperature stress. The NOS activity and NO concentration increased significantly after heat stress. How ever,ATP concentration increased significantly after both low temperature and high temperature stress,and it returned to the control level after 24 h recovery. Conclusion：Thermal stress could lead to oxygen metabolism disorder in the hepatopancreas of shrimp L. vannamei,w hich might induce antioxide enzymes response. Our findings suggested that SOD,CAT and GSH might play different roles in shrimp response to thermal stress. Also,NO might play an important role in induction of many signaling pathw ays to response to thermal stress. In addition,more ATP was produced after cold and heat stress. Our findings indicated that thermal stress lead to oxygen metabolism disorder,which might be due to the temperature stress beyond the oxygen and capacity limited thermal tolerance（ OCLTT） of L. vannamei.The disorder of oxygen metabolism might have an important impact on energy metabolism and other physiological