中文摘要：

以来源于多种组织的mRNA为模板,构建了-个凡纳滨对虾混合组织的T 7噬菌体cDNA展示 文库.结果表明噬菌体文库容量大,达9.3 ×10^7pfu;文库重组率髙,为97 %;大于250 bp的片段占70 %以上;利用液体扩增法对文库进行扩增,扩增后文库的滴度为2. 1×10^12 pfu/mL.

英文摘要：

In order to investigate the gene function and protein interaction, total RNA were extracted from various tissues of Litopenaeus vannamei and then pooled together. The mRNA was separated from the pooled total RNA and a T7 phage display cDNA library of L. vannamei was constructed. Results showed that the total number of primary recombinants of the T7 phage display cDNA library was about 9. 3 × 10^7 pfu, the recombination ratio was 97% and the ratio of fragments greater than 250 bp was proved to be 7 0 % by PCR amplification. The phage titer of the amplified library was 2. 1 ×10^12 pfu/mL. In general, a T7 phage dis-play cDNA library from L. vannamei was successfully constructed with a large reservoir capacity and high re-combination rate, which is helpful for the investigation of gene function and protein interaction.