我们测试了在少年黑人各种栖于礁石中的鱼类 Sebastes schlegelii 标记技术的化学药品的用途。少年( 3040 公里总数长度)沉浸于四圜素 hydrochloride ( TC )的一个范围在从 300~500 mg/L 的集中的答案,和在在过滤的海从 200~400 mg/L 的集中的茜素 complexone ( ALC )答案流水( 30 的咸度)为 24 h ,分别地。耳石(sagittae, asteriscus ) ,规模,鳍光线(背面、胸、腹、肛门、尾的鳍光线) ,和鳍脊骨(背面、腹、肛门的鳍脊骨) 被取样并且过去常检测荧光灯在一个 60 天的生长实验以后的标记。与 300 mg/L TC 的例外,可接受的标记被 TC 和 ALC 的所有集中在耳石和鳍脊骨生产。特别地,我们分别地在 200400 mg/L, 250400 mg/L,和 250400 mg/L ALC 的集中在正常的光下面在 sagittae, asteriscus,和鳍脊骨观察了清楚地可见的标记。规模和鳍光线在高得多的集中有可接受的标记(350 mg/L TC,为规模的 250 mg/L ALC 和 350 mg/L TC,为鳍光线的 300 mg/L ALC ) 。最好的标记质量(即,可接受的标记在沉浸标记以后在所有取样的结构被观察) 被获得在 TC 跟随沉浸在在在 300400 mg/L 之间的 350500 mg/L,和 ALC 之间。另外,与他们直到邮寄的 60 天的控制相比在 TC 和 ALC 显著的鱼的幸存和生长没有重要差别(P > 0.05 ) 。
We tested the utility of chemical marking techniques in the juvenile black rockfish Sebastes schlegelii. Juveniles (30-40 mm total length) were immersed in a range of tetracycline hydrochloride (TC) solutions at concentrations ranging from 300 to 500 mg/L, and alizarin complexone (ALC) solutions at concentrations ranging from 200 to 400 mg/L in filtered sea water (salinity of 30) for 24 h, respectively. Otoliths (sagittae, asteriscus), scales, fin rays (dorsal, pectoral, ventral, anal, and caudal fin rays), and fin spines (dorsal, ventral, and anal fin spines) were sampled and used to detect fluorescent marks after a 60-day growth experiment. With the exception of 300 mg/L TC, acceptable marks were produced in the otoliths and fin spines by all concentrations of TC and ALC. In particular, we observed clearly visible marks in the sagittae, asteriscus, and fin spines under normal light at concentrations of200~00 mg/L, 250-400 mg/L, and 250-400 mg/L ALC, respectively. Scales and fin rays had acceptable marks at much higher concentrations (_〉350 mg/L TC, 〉250 mg/L ALC for scales and _〉350 mg/L TC, 〉300 mg/L ALC for fin rays). The best mark quality (i.e., acceptable marks were observed in all sampled structures after immersion marking) were obtained following immersion in TC at between 350-500 rag/L, and ALC between 300-400 mg/L. In addition, there was no significant difference in survival and growth of TC and ALC marked fish compared to their controls up to 60 days post-marking (P〉0.05).