中文摘要：

微量热泳动技术基于荧光标记分子在温度梯度内的定向移动，能够在溶液中快速、灵敏地测量生物相关分子间的相互作用。它不需要表面固定和大量样品，对相对分子质量也没有限制。热泳动对分子的大小、电荷和水化层的微小变化都十分敏感，适合于蛋白质、核酸、小分子和离子等多种物质的相互作用研究。微量热泳动技术由于其独特的优势，目前已经在生物医学领域中有了初步应用。本文介绍了微量热泳动技术的背景和其在生物医学领域中不同生物相关分子间相互作用分析的研究进展。

英文摘要：

Microscale thermophoresis （MST） technology is based on the directed movements offluorescently labeled molecules in a temperature gradient, and it can quickly and sensitively quantify biologically related molecule interactions in free solutions. It doesn＇t need surface immobilization and high sample consumption, and has no limitation with molecular weight. Thermophoresis is sensitive to the tiny changes of molecular size, charge and hydration shell, and is suitable for the biologically related molecule interactions research, such as protein, nucleic acids, small molecules, ions and other chemicals. Because of its unique advantages, MST has primarily applied in biomedical field. In this review, we present the background, progress and devel- opments of MST in the biologically related molecule interactions in biomedical fields.