中文摘要：

建立一种同时测定杨树花和杨树叶中豆甾醇、麦角甾醇、β-谷甾醇含量的反相高效液相色谱法。方法：以甲醇为流动相,测定杨树花和杨树叶中豆甾醇、麦角甾醇、β-谷甾醇的含量。结果：杨树花和杨树叶中3个植物甾醇的线性范围为0.00008-0.08μmol·m L-1,r〉0.9990。杨树花中麦角甾醇、豆甾醇、β-谷甾醇的加样回收率分别在92.6%-100.0%、93.9%-100.1%、94.0%-100.0%之间,RSD（n=6）分别为2.8%、2.2%、2.8%;杨树叶中各甾醇加样回收率分别在91.3%-100.0%、91.2%-100.0%、92.5%-100.1%之间,RSD（n=6）分别为2.7%、2.7%、2.5%。结论：该方法稳定、快速,精密度高,线性范围宽,适合杨树花和杨树叶中的豆甾醇、麦角甾醇、β-谷甾醇含量测定。

英文摘要：

Objective： To establish a method for determination of the contents of three phytosterols（stigmasterol, ergosterol,β- sitosterol）from Flos Populi Canadensis and leaves of Populus canadensis Moench by RP- HPLC. Methods： With methanol as the mobile phase,HPLC method was adopted to determine contents of phytosterols in Flos Populi Canadensis and leaves of Populus canadensis Moench. Results： The three phytosterols had good linearity in their ranges of 0. 00008 - 0. 08 μmol · mL- 1 （ the coefficients of correlation were all over 0. 9990）. The average recoveries of stigmasterol ,ergosterol β- sitosterol in Flos Populi Canadensis were 92.6% - 100.0% ,93.9% - 100. 1% ,94.0% - 100. 0% ,and RSDs（n =6）were 2. 8% ,2.2% ,2. 8% ,respectively;the av- erage recoveries（ n = 6）of the three phytosterols in leaves of Populus canadensis Moench were 91.3% - 100.0% , 91.2% - 100. 0% ,92.5% - 100. 1% ,and RSDs were 2.7% ,2.7% ,2.5% ,respectively. Conclusion ： The method is sensitive, accurate and reproducible, and reliable for the determination of phytosterols in Flos Populi Canadensis and leaves of Populus canadensis Moench.