中文摘要：

为了研究阉割对甲状腺转录因子-1、2（TTF-1,2）基因的影响,本研究采用荧光定量PCR技术分析阉割与非阉割金华猪甲状腺组织中TTF-1和TTF-2基因在60、90和120日龄时的表达水平。结果表明：非阉割猪TTF-1和TTF-2基因在不同生长阶段的表达量呈稳步上升趋势,阉割猪TTF-1和TTF-2基因的表达基本稳定。TTF-1与TTF-2mRNA的表达量均是在60、90日龄时阉割组高于非阉割组;在120日龄时,阉割组TTF-1与TTF-2mRNA的表达量均低于非阉割组,但差异不显著（P〉0.05）。结果,建立的SYBR-Green荧光定量PCR方法可以有效地用于TTF-1和TTF-2基因的表达分析;另外,阉割使得猪甲状腺TTF-1和TTF-2基因在60、90日龄的表达量升高,且TTF-1基因的表达与猪部分肉质性状存在显著的相关性。

英文摘要：

In order to investigate the effect of castration on expression of TTF-1 and TTF-2 genes,real-time fluorescence quantitative PCR was applied to investigate the developmental expression patterns of TTF-1 and TTF-2 genes from the thyroid tissue of castrated and uncastrated Jinhua pigs on the age of 60,90 and 120 days.The results showed that the expression of TTF-1 and TTF-2 genes increased slightly in uncastrated pigs,while they were relatively stable in castrated pigs.The expression level of TTF-1 and TTF-2 mRNA in castrated Jinhua pigs on 60 and 90 days were higher than that of uncastrated pigs,while the expression level of TTF-1 and TTF-2 on 120 days were lower than that of uncastrated pigs,but no significant difference（P0.05）.The results indicated that the real-time PCR method was faithful for detecting the expression analysis of TTF-1 and TTF-2 genes,and the expression of TTF-1 and TTF-2 mRNA increased after castration.Furthermore,there was a significant correlation between TTF-1 gene expression and meat quality traits.