中文摘要：

目的 建立人类淋巴瘤B JAB多药耐药细胞株BJAB/ADR,并初步研究其耐药机制.方法 采用多柔比星浓度梯度递增法建立人类B细胞淋巴瘤耐药细胞模型BJAB/ADR,观察其生长规律并绘制细胞生长曲线;用四甲基偶氮唑蓝（MTT）法鉴定耐药细胞株对多种化疗药物的耐药性并计算耐药指数;提取耐药细胞株RNA,实时定量聚合酶链反应（PCR）法检测相关耐药基因MDR1 mRNA的表达;流式细胞术检测细胞表面P糖蛋白（Pgp）的表达;通过罗丹明外排实验,检测Pgp功能.结果 成功建立B细胞淋巴瘤耐药细胞模型BJAB/ADR,并在160 ng/ml多柔比星溶液中稳定生长,耐药细胞较敏感细胞生长缓慢,细胞形态无明显变化.MTT检测结果表明BJAB/ADR细胞对多柔比星的耐药指数为43倍,同时对柔红霉素、依托泊苷、高三尖杉酯碱（HHT）及米托蒽醌（MX）也具有一定的耐药性.实时定量PCR结果表明,耐药细胞BJAB/ADR MDRl mRNA明显高于药物敏感细胞BJAB（P＜ 0.01）.流式细胞术检测结果显示,耐药细胞表面Pgp高表达;罗丹明外排实验表明Pgp对多柔比星具有外排功能,使B JAB/ADR细胞获得耐药特性.结论 建立了人类B细胞淋巴瘤的耐药细胞株BJAB/ADR,其对多柔比星耐药性稳定,并呈现多药耐药细胞的基本生物学特性,为进一步研究肿瘤多药耐药发生的机制及逆转耐药提供有利的模型.

英文摘要：

Objective To establish human multi-drug resistance （MDR） B-cell lymphoma cell line BJAB/ADR and investigate its biological characteristics.Methods Human B-cell lymphoma MDR cell line BJAB/ADR was induced by exposure to increasing dose of adriamycin.The growing features of BJAB/ADR were observed and cell growth was measured,IC50 of antitumor agents was evaluated by MTT assay.The expression of MDR1 mRNA was determined with real-time PCR,FACS was applied to study the expression of MDR protein P-glycoprotein （Pgp） and Rhodamine 123R efflux assay was used to determine the function of Pgp.Results Human B-cell lymphoma MDR cell line BJAB/ADR was successfully established.MTF results showed BJAB/ADR grew steadily with 160 ng/ml adriamycin in the medium and possessed 45-fold resistance to adriamycin compared with BJAB.Also BJAB/ADR cells exhibited cross-resistance to daunorubicin,etoposide,homohardngtonine and mitoxantrone.In term of the cell proliferation,as compared to BJAB cells,BJAB/ADR cells grew more slowly without obvious changes of cellular morphology.QRT-PCR indicated that the mRNA level of MDR1 in BJAB/ADR resistant cells was significantly higher than that in BJAB cells （P 〈 0.01）.FACS results revealed that the protein expression of Pgp in BJAB/ADR was increased compared with that in BJAB cells.Rhodamine 123R efflux assay indicated Pgp had the capacity of drug excretion and decreased the intracellular drug concentration.Conclusions Human B-cell lymphoma MDR cell line BJAB/ADR is successfully established.BJAB/ADR possesses a stable adriamycin resistance and presents some basic biological characteristics of MDR cells.It offers a model with MDR phenotype for the study of MDR in human B-cell lymphoma.