中文摘要：

目的 观察Notch1和JAG1在乳腺癌中的表达及其与乳腺癌相关因素的关系，探讨Notch信号在人类乳腺癌中的作用。方法应用逆转录聚合酶链反应（RT—PCR）检测62例乳腺癌组织及22例癌旁正常乳腺组织中Notch1和JAG1的表达，对乳腺癌组织与癌旁正常乳腺组织进行表达率和表达强度标准化系数的统计学比较，并在不同的腋窝淋巴结转移情况间、不同的临床分期间进行Notch1表达强度标准化系数的统计学比较。结果乳腺癌组织Notch1的表达率和标准化系数分别为98．0％和0．91，均明显高于癌旁正常乳腺组织，乳腺癌组织中JAG1的表达率为15．0％，癌旁组织中无JAG1表达；伴腋窝淋巴结转移的病例Notch1标准化系数高于无腋窝淋巴结转移的病例；乳腺癌Ⅰ期病例Notch1标准化系数（0．66）低于Ⅱ期（1．20），Ⅱ期高于Ⅲ期（0．62），Ⅰ期与Ⅲ期差异无统计学意义。结论 人类乳腺癌中存在Notch1和JAG1的异常高表达，提示Notch1的异常表达与活化可能与人类乳腺癌的形成有关，Notch1在人类乳腺癌不同发展阶段的作用可能不同。

英文摘要：

Objective To explore the role of Notch signaling in human breast cancers, the expression of Notchl and its ligand JAG1 in human breast cancers and their relationships with clinical stages of breast cancers were analyzed. Methods RT-PCR was used to detect the expression of Notchl and JAG1 in 62 breast cancer specimens and 22 normal breast tissues at the margin of tumor sections, and the statistical difference of expression rates and standardized coefficient between the two groups were analyzed. To compare the expression intensity of Notchl and JAG1 at different development stages of the illness and at different stages with or without axillary node metastasis. Results The expression rate and standardized coefficient of Notchl in human breast cancers were significantly higher than those of normal breast tissues at the margin of tumor sections. The expression rate of JAG1 in human breast cancers was 15% , while JAG1 was not detected in normal breast tissues at the margin of tumor sections. The standardized coefficient of Notchl in cases with axillary node metastasis was significantly higher than that in cases without axillary node metastasis. The standardized coefficient of Notchl at stage Ⅰ was significantly lower than that at stage Ⅱ , and stage Ⅱ was significantly higher than stage Ⅲ. There was no statistically significant difference between stage Ⅰ and stage Ⅲ. Conclusion Notchl and JAG1 are highly expressed in human breast cancers, indicating that the aberrant expression and activation of Notchl may be related with tumorigenesis of human breast cancer. Notchl may play different roles at different developmenfl stages of human breast cancer.