中文摘要：

遗传上修改的蚊子的使用减少或代替地人口是新策略控制忍受蚊子的疾病。这策略的实现的前提是操作蚊子的染色体并且导致一个合适的倡导者驾驶的受动器分子的特定的表示的能力。这研究的目的是在转基因的疟蚊属 stephensi 在 avitellogenin 倡导者的控制下面计算疟蚊属 sinensis 的 defensin 三氯化氮的表达式。疟蚊属 gambiaevitellogenin 的规章的区域被克隆，潜水艇克隆进与编码顺序的 defensin A 由一个表示盒子组成的转移向量 pSLFa。然后，表示盒子被变成转变向量 pBac [3xP3-DsRedafm ] 用 Asc l 消化。recombinant 原生质标志 DNA ofpBac [3xP3DsRed-AgVgT2-DefA ] 并且助手原生质标志 DNA ofphsp-pSac 是进胚胎的微注射一。Stephensi。积极转基因的蚊子被在官方补给的幼虫的心中观察特定的红荧光屏蔽。南部的污点分析显示出那单个拷贝的 transgeneintegrated 进染色体一。Stephens ! 。RT-PCR 分析证明 defensin A 基因在血饭以后在女蚊子的胖身体明确地表示了。有趣地， mRNAof defensin A 与 endogenousvitellogenin 基因的相比是更稳定的。在多重血饭以后， defensin A 的表示作为一种可约的骑车 andnon 类型出现了，为它的反病原体效果的一个关键特征。从上面的数据，我们断定 Vg 倡导者的规章的函数和 defensin 的表达式基因相对在疟蚊蚊子的不同种类被保存。这些分子能在遗传上修改的蚊子的发展被用作候选人。

英文摘要：

The use of genetically modified mosquitoes to reduce or replace field populations is a new strategy to control mosquito-borne diseases. The precondition of the implementation of this strategy is the ability to manipulate the genome of mosquitoes and to induce specific expression of the effector molecules driven by a suitable promoter. The objective of this study is to evaluate the expression of defensin A gene of Anopheles sinensis under the control of a vitellogenin promoter in transgenic Anopheles stephensi. The regulatory region of Anopheles gambiae vitellogenin was cloned and subcloned into transfer vector pSLFa consisting of an expression cassette with defensin A coding sequence. Then, the expression cassette was transferred into transformation vector pBac[3xP3-DsRedafm] using Asc I digestion. The recombinant plasmid DNA of pBac[3xP3DsRed-AgVgT2-DefA] and helper plasmid DNA of phsp-pBac were micro-injected into embryos of An. stephensi. The positive transgenic mosquitoes were screened by observing specific red fluorescence in the eyes of G1 larvae. Southern blot analysis showed that a single-copy transgene integrated into the genome of An. stephensi. RT-PCR analysis showed that the defensin A gene expressed specifically in fat bodies of female mosquitoes after a blood meal. Interestingly, the mRNA of defensin A is more stable compared with that of the endogenous vitellogenin gene. After multiple blood meals, the expression of defensin A appeared as a reducible and non-cycling type, a crucial feature for its anti-pathogen effect. From data above, we concluded that the regulatory function of the Vg promoter and the expression of defensin A gene were relatively conserved in different species of anopheles mosquitoes. These molecules could be used as candidates in the development of genetically modified mosquitoes.