中文摘要：

目的 克隆中华按蚊防御素基因全长cDNA序列及基因组序列，并对其进行鉴定和生物信息学分析。方法 根据已发表的埃及伊蚊和冈比亚按蚊等的防御素基因序列设计引物，提取中华按蚊总RNA并构建其基因组文库，分别进行多轮RT-PCR和巢式PCR扩增，将所得片段进行克隆、测序，并应用相关生物信息学软件对序列进行鉴定和分析。结果 从中华按蚊基因组文库中扩增出完整的防御素基因组序列（由两个外显子和一个内含子组成）以及5’端和3’端的非编码序列（UTR）片段，总长度为2256bp；从中华按蚊总RNA中扩增出大小为324bp的cDNA片段，经测序证实为中华按蚊防御素基因全长cDNA序列，其开放阅读框共编码107个氨基酸，成熟肽部分具有40个氨基酸残基。结论 首次克隆出中华按蚊防御素基因全长cDNA序列及基因组序列，为进一步的功能研究奠定了基础。

英文摘要：

Objective To clone and identify the cDNA sequence and genomic DNA sequence of Anopheles sinensis defensin gene. Methods Referring to the published defensin gene sequences of Aedes aegypti and Anopheles gambiae, pairs of primers were designed to amplify the cDNA sequence and genomic DNA sequence of Anopheles sinensis defensin gene with template of Anopheles sinensis total RNA by RT-PCR and genomic DNA by nested PCR, respectively. These amplified fractions were cloned and sequenced, and were further identified and analyzed by relevant bioinformatics softwares. Results Complete genomic DNA sequence was cloned, including 5＇ and 3＇ UTR fraction and two exons separated by a 85 bp intron. Besides, the whole cDNA sequence of Anopheles sinensis defensin gene with 324 bp was also cloned, and its ORF encoded 107 amino acids. The mature peptide had 40 amino acids residues. Conclusion The whole cDNA sequence and complete genomic DNA sequence of the defensin gene of Anopheles sinensis have been cloned and identified for the first time.