中文摘要：

瞄准：像马球的激酶 1 (PLK1 ) serine/threonine 激酶在胃的癌症房间在有丝分裂的多重阶段起一个重要作用。在胃的癌症房间的有丝分裂和 apoptosis 上调查 PLK1 弄空的效果。方法：PLK1 表示被小 RNA 干扰(siRNA ) 堵住。PLK1， cdc2， cyclin B 和 caspase 的表示层次 3 被西方的弄污检测。然后， PLK1 击倒的房间的 apoptosis 的 PLK1 弄空， cdc2 活动，房间增长，房间周期阶段分发，有丝分裂的锭子结构，和率被观察。结果：PLK1 基因击倒与增加的 cyclin B 表示被联系，增加的 cdc2 活动(然而并非与表示层次) ，在 G2/M，不适当的有丝分裂的锭子形成，推迟的染色体分离和推迟或逮捕的胞质分裂的胃的癌症房间的累积。而且，在胃的癌症房间的 PLK1 弄空与减少的增长， 3 铺平的稀释 pro-caspase 和增加的 apoptosis 被联系。结论：PLK1 表示的阻塞可以在胃的癌症房间导致减少的有丝分裂或甚至 apoptosis，显示 PLK1 可以是为胃的癌症的一个珍贵治疗学的目标。

英文摘要：

AIM： Polo-like kinase 1 （PLK1） serine/threonine kinase plays a vital role in multiple phases of mitosis in gastric cancer cells. To investigate the effect of PLK1 depletion on mitosis and apoptosis of gastric cancer cells. METHODS： PLK1 expression was blocked by small RNA interference（siRNA）. The expression levels of PLK1, cdc2, cyclin B and caspase 3 were detected by Western blotting. Then, PLK1 depletion, cdc2 activity, cell proliferation, cell cycle phase distribution, mitotic spindle structure, and the rate of apoptosis of the PLK1 knockdown cells were observed. RESULTS： PLK1 gene knockdown was associated with increased cyclin B expression, increased cdc2 activity （but not with the expression levels）, accumulation of gastric cancer cells at G2/M, improper mitotic spindle formation, delayed chromosome separation and delayed or arrested cytokinesis. Moreover, PLK1 depletion in gastric cancer cells was associated with decreased proliferation, attenuated pro-caspase 3 levels and increased apoptosis. CONCLUSION： Blockage of to decreased mitosis or even PLK1 expression may lead apoptosis in gastric cancer cells, indicating that PLK1 may be a valuable therapeutic target for gastric cancer.