中文摘要：

应用改良的剪切消化法（1%Ⅳ型胶原酶）消化分离仔猪肝脏细胞,建立稳定的仔猪肝脏细胞的分离与原代培养体系;通过siRNA途径及RT-PCR技术检测肝细胞THRSP基因mRNA表达水平。结果表明,平均每g肝脏可获得6.2×10^6个细胞,接种即时存活率达94.84%,培养存活率达50%左右,细胞转染后细胞存活率达80%以上;设计合成的siRNA的干扰效率为75.3%

英文摘要：

In this study, digesting and separating piglets liver cells obtained from modified shear digestion （1% Ⅳcollagenase ）were cultured and dynamically observed with inverted microscopy, so as to establish a stable system for primary culture of piglets liver cells. RNAi and RT-PCR technique were employed to the detection of the cell mRNA expression level, and then the changes of THRSP mRNA expression at the cellular level were explored. The results showed that 6.2×10^6 cells can be obfained from per gram of liver on the average, with seeding survival rate of 94.84% and the culture survival rate of about 50%, and transfected cell viability was more than 80%. The interfered efficiency of chemosvnthetic siRNA was 75.3%.