中文摘要：

聚合酶链式反应（PCR）是一种高灵敏核酸扩增技术,广泛应用于核酸检测中。但在实际应用过程中,扩增产物及其他核酸片段的污染会导致假阳性的结果,制约了PCR在临床检测中的应用。为了解决这一问题,建立了许多PCR防污染的方法,除了早期建立的并已得到广泛应用的物理隔绝法、光照法及水解法外,近年来还发展了酶消化法、化学修饰法及DEAE纤维素法。本文对PCR防污染技术的原理、应用及进展进行了综述。

英文摘要：

Polymerase chain reaction（PCR） is a highly sensitive nucleic acid amplification technique,which has been widely used in nucleic acid detection.However,the contamination of amplicons or other nucleic acid fragments,which usually leads to false-positive results,limits it＇s clinical applications.To solve this problem,many approaches were developed to eliminate PCR contami-nation.Besides early established methods based on physical separation,irradiation and hydrolysis,methods by using enzyme digestion,chemical modification and DEAE cellulose are developed recently.In this paper,the principles,applications and advances of PCR decon-tamination methods are reviewed.