中文摘要：

钙调素（CaM）是植物钙离子信号通道的主要参与者。参与低温胁迫下多种植物的抗寒生理作用。本研究根据钙调素基因相关表达序列标签（EST）序列，借助RACE—PCR技术，获得CsCaM1和CsCaM2两条cDNA全长序列。GenBank登录号分别为KT238971和KT238972，长度分别为693bp和841bp，均包含450bp的完整开放阅读框（ORF），编码149个氨基酸，两条氨基酸序列仅一个氨基酸有差异，且均含有4个植物CaM家族的共同特征手型结构EF（EF-hand）。采用实时荧光定量PCR（qRT-PCR）分析CsCaMs在茶树低温胁迫下各种处理中的表达模式。结果表明，CsCaMs无组织表达特异性，低温胁迫处理和CaCl，均能诱导D饧胁的表达，而钙调素拮抗剂W7与钙离子通道抑制剂LaCl3则会抑制其表达。本研究结果对阐明茶树抗寒性的分子机理有一定理论意义，为茶树的抗寒性育种提供参考。

英文摘要：

Calmodulin（CaM） is a major participant in calcium signaling pathway, involved in multiple physiological processes of plant under cold stress. Using RACE-PCR and RT-PCR, two full-length cDNAs of CsCaM were cloned from tea plant based on the available EST sequences. CsCaM1 had 693 bp in length, and CsCaM2 had 841 bp in length. Both of them contained a 450 bp open reading frame（ORF） encoding 149 amino acid residues. Cs- CaMs had four EF-hands, which were CaM gene families＇ characteristic. CsCaM1 and CsCaM2 were submitted to GenBank with accession number of KT238971 and KT238972, respectively. The expression profiles of CsCaMs under cold treatment were investigated using quantitative real-time PCR. Results indicated that the expressions of CsCaMs were induced gradually with the extension of cold treatment. Under CaCl2 treatment, the expressions of CsCaMs were up-regulated. However,the expressions of CsCaMs were down-regulated under W7 and LaCl3 treatments. Above results demonstrated that CsCaMs might play an important role in cold tolerance of tea plant.