中文摘要：

目的：提高核糖体基因区打靶载体的转染效率,优化其电转染肝细胞的孵育条件。方法：在其它电转染参数一致的前提下,设计了6组不同的孵育条件,通过分析转染后细胞活率以及48h后目的基因GFP的表达效率,来比较多组不同的孵育条件对电转染效率的影响。结果：6组条件中,转染前21℃,1min,转染后21℃,1min孵育,得到的转染效率最优,比其它的条件得到的转染效率高50%。结论：孵育条件的优化能提高为核糖体基因区靶向表达载体在体外电转染肝细胞的转染效率。

英文摘要：

Objective：To explore the optimal incubation parameters in the electroporation for the in vitro introduction of hrDNA（human ribosomal DNA） targeting vector into hepatocyte,to improve the transfection efficiency.Methods：Six different incubation conditions were designed,in which all other parameters were identical.The cell survival rates after electroporation and the GFP gene-expressing cell rates 48h after the electroporation were calculated to analyses the effect of different incubation conditions.Result：Among the 6-group incubation conditions,highest transfection efficiency was shown in group with 21℃,1min incubation before electroporation and group with 21℃,1min incubation after electroporation,which were 50% higher than the others.Conclusion：Optimization of incubation condition improved the transfection efficiency of hrDNA targeting vector into hepatocyte.