中文摘要：

目的：研究胎膜来源的间充质干细胞在治疗SD大鼠Ⅲ度烧伤中的作用。方法：用胰酶消化法分离足月产胎膜细胞,加入Amino-MAXⅡ培养基进行培养。流式细胞仪检测细胞表面标志物CD29、CD73、CD14、CD45。分别用浓度为3mg/ml、5mg/ml、7mg/ml、9mg/ml的明胶溶液包被脱细胞真皮（ADM）,以5×106/ml的密度将细胞种植在包被和未包被的ADM表面,2小时后收集未贴附在ADM上的细胞,计算细胞种植效率。实验组：以7mg/ml的明胶包被ADM,以5×106/ml的密度种植第2～3代细胞,培养3～5天后,移植至SD大鼠Ⅲ度烧伤创面;对照组Ⅰ移植单纯的ADM,对照组Ⅱ未进行移植,术后定期观察创面大体情况,计算创面收缩率、表皮再生面积比例,第5周切取新生皮肤组织行HE染色。结果：胎膜分离的细胞以长梭形为主,表达与间充质干细胞相关的表面抗原标志物CD29、CD73,极少表达造血细胞标志物CD14、CD45。不同浓度明胶溶液包被和未包被的ADM,其细胞种植效率均无明显区别（P〉0.05）;相比对照组,实验组移植物成活时间更长,创面再上皮化时间较早,创面收缩率较小,再生表皮面积亦较多（P〈0.05）;再生表皮更厚,有明显的表皮突,真皮层毛细血管丰富。结论：成功分离、培养了胎膜来源的间充质干细胞。胎膜来源间充质干细胞复合ADM可促进SD大鼠Ⅲ烧伤创面表皮再生,加速创伤愈合,抑制创面收缩。

英文摘要：

Objective：To assess the effect of fetal membrane-derived mesenchymal stem cells（MSCs） on full deep burn healing in rat.Methods：The term fetal membranes were treated with trypsin solution and the derived cells were cultivated with Amino-MAX Ⅱ media.The acelluar dermal matrix（ADM） was coated with different concentration of gelatin solutions as 3mg/ml,5mg/ml,7mg/ml and 9mg/ml.Cell solutions at 5×106/ml were seeded on coated and uncoated ADM respectively.Two hours later,the cells inadhere to the ADM were collected to calculate the efficiency of cell seeding.In experimental group,the cells（Passage 2 to 3） solutions at 5×106/ml were seeded on ADM coated by 7mg/ml gelatin,cultured for 3 to 5 days and then transplanted to the SD rat full-thickness burn wound.Control group I was transplanted with the ADM without cells and control group Ⅱ was not transplanted with ADM.The whole wound surface,wound contraction and the ratio of reepithelization area were calculated regularly.Tissue samples were harvested for histological examination in the fifth week.Results：Cells from fetal membranes were splindle-like in the majority,positive for the MSC surface antigen markers CD29 and CD73 and negative for those of hematopoietic cell CD14 and CD45.The coated and uncoated ADM with different concentration gelatin solutions didn＇t show statistical difference in the efficiency of cells seeding.Compared with control group,experimental group had longer transplant survival,earlier reepithelization,smaller contraction of wound surface and larger reepithelization area.Thicker regenerated epidermis and more apparent cuticular and capillary process were also observed in experiment group.Conclusion：MSCs from fetal membranes were successfully separated and cultured.Combining with ADM,the MSCs induced epidermis regeneration,accelerated the burn wound healing and inhibited the contraction of wound surface in the SD rats full-thickness burn wound model.