中文摘要：

目的探索小鼠在其早期发育阶段暴露不同浓度亚砷酸钠后,各砷形态在肝和脑组织中的代谢与分布情况。方法母鼠在妊娠和哺乳期以自由饮水方式暴露0、10和30 mg/L iAsⅢ水溶液,仔鼠在哺乳期后继续摄入与母鼠相同浓度的含砷水溶液。分别在仔鼠出生后第0、10、15、21和35天,采用氢化物发生-超低温捕集-原子吸收分光光度法测定肝和脑组织中无机砷（iAs）、一甲基胂（MMA）和二甲基胂（DMA）水平。结果从出生后15 d起,肝组织中iAs含量开始逐步增加;从出生后21 d起,肝组织中MMA含量开始逐步增加;肝组织中DMA含量在出生后10～15 d最低,以后逐步增高。脑组织中iAs含量,在出生后15 d开始升高,在出生后21 d时达最高水平;MMA含量在早期发育阶段的脑组织中没有检测到;DMA含量在出生后10～15 d最低。结论母鼠体内各形态砷化物可通过胎盘屏障进入胎鼠体内,但其基本不能通过乳房屏障进入母鼠乳汁,进而进入仔鼠体内,成熟的血脑屏障对iAs具有一定的阻挡作用,但可允许部分DMA进入脑组织。

英文摘要：

Objective To explore the metabolism and distribution of different speciations of arsenic in liver and brain of baby-mice exposed to arsenite during early developmental stage.Methods Mother mice were exposed to iAsⅢ through drinking water during gestation and lactation,the concentrations of iAsⅢ in the water were 0,10 and 30 mg/L.After birth,the baby-mice were also given same water.At the 0,10,15,21 and 35 postnatal days（PND）,the level of inorganic arsenic（iAs）,monomethylarsonic acid（MMA）and dimethylarsinic acid（DMA）in both liver and brain of the baby-mice were determined by hydride generation-atomic absorption spectrometry（HG-AAS）.Results In liver,level of iAs increased from PND 15,and level of MMA increased from PND 21,however,level of DMA showed some lower between PND 10 and 15.In brain,level of iAs increased from PND 15,the peak was on PND 21;level of MMA was undetected during the early developmental stages;level of DMA was also showed a valley between PND 10 and 15.Conclusion It was suggested that various speciations of arsenicals might enter into the body of pregnant mice and permeate through transplacental barrier,basically they could not permeate through lactational barrier then get into the body of baby-mice via milk of mother mice;additionally,well-developed blood-brain barrier has certain hindrance to iAs,but some DMA could still enter into brain.