中文摘要：

目的探讨亚急性砷暴露对小鼠海马各区星形胶质细胞的影响。方法将昆明种小鼠随机分为对照组及低、中和高剂量染毒组。以自由饮水方式饮含砷水,对照组小鼠饮蒸馏水。染毒8周后,心脏灌流固定,取大脑,进行胶质纤维酸性蛋白（GFAP）免疫组化染色,分析砷对小鼠海马各区GFAP表达情况,判断其对星形胶质细胞的影响。结果雌鼠海马CA1、CA3和DG区的GFAP积分光密度（IOD）值均先升高而后下降;在海马CA1和DG区,低剂量组的IOD值最高,并显著高于对照组,而高剂量组的IOD值则显著低于对照组;但在海马CA3区,低、中剂量组的IOD值均显著高于对照组,以中剂量组的IOD值最高,而高剂量组则显著低于对照组。雄鼠海马CA1和DG区GFAP的IOD值也先升高而后下降,中剂量组的IOD值最高,并显著高于对照组;但在海马CA3区,IOD值随染砷剂量的增加不断增高,高剂量组的IOD值最高,并显著高于对照组。结论低水平砷暴露可使小鼠海马星形胶质细胞活化,而高水平砷暴露会损伤海马星形胶质细胞;雌鼠海马星形胶质细胞对砷作用比雄鼠敏感;而无论雌、雄鼠,其海马CA1和DG区星形胶质细胞对砷的作用比CA3区敏感。

英文摘要：

Objective To explore the effect of arsenic on astrocyte in the hippocampal CA1,CA3 and DG of mice.Methods Mice were randomly divided into four groups,which were control group,low dose group,medium dose group and high dose group.Mice were exposed to arsenite through drinking water for eight weeks.Expression of glial fibrillary acidic protein（GFAP）in hippocampal CA1,CA3 and DG of mice was determined by immunohistochemical staining method.Results In hippocampal CA1,CA3 and DG of female mice,the integral optical density（IOD）of GFAP was increased and then decreased along with the arsenic dose;IOD in CA1 and DG of low dose group was the highest and significantly higher than that in control;but was significantly lower in high dose group than that in control;however,IOD in CA3 of medium dose group was the highest,and was significantly higher in both low and medium dose group than that in control,but was significantly lower in high dose group than that in control.In hippocampal CA1 and DG of male mice,IOD of GFAP was increased and then decreased;IOD in medium dose group was the highest and significantly higher than that in control;however,IOD of GFAP in CA3 male mice was increased along with arsenic dose,which was the highest in high dose group and significantly higher than that in control.Conclusions Exposure to low arsenic levels could activate astrocytes,however high levels of arsenic could injure astrocytes.Compared to that in male mice,astrocytes in hippocampus of female mice are more sensitive to the effects of arsenic.Effects of arsenic on astrocytes in CA1 and DG seemed to be more stronger than in CA3.