中文摘要：

目的了解嗜麦芽寡养单胞菌（SMA）的耐药特征，整合酶（Int）基因、qacE△1-sull基因及整合子携带的耐药基因盒的存在情况。方法采用琼脂倍比稀释法测定6种药物对83株嗜麦芽寡养单胞菌的最低抑菌浓度；PCR扩增qacE△1-sull基因、intl、intlI、intⅢ及I类整合子可变区。结果83株嗜麦芽寡养单胞菌对头孢他啶、替卡西林／克拉维酸的耐药率较高，分别是63．8％和1175．9％，对磺胺甲嗯唑／甲氧苄啶的耐药率为31．3％；检出18株qacE△1-sull基因阳性株，20株intI阳性株，I类整合子可变区检出3类基因盒，aacA4-catB8-aadAl、aac（6＇）-Ⅱ-6blaCARB-8aar-3-dfrA27基因盒，首次在SMA中发现aar-3-dfrA27基因盒，GenBank登录号为KC748137。结论本次研究的嗜麦芽寡养单胞菌耐药情况严重，临床上应在药敏结果指导下合理使用抗菌药物。SMA对磺胺甲噫唑／甲氧苄啶的耐药性增加可能与I类整合子有关，整合子的存在加快了细菌耐药的传播。

英文摘要：

Objective To investigate the characteristics of antimicrobial resistance, the distribution of integrons and gene cassettes embedded in clinical Stenotrophomonas maltophilia isolates. Methods Minimal inhibitory concentrations of six antimicrobial agents was determined by agar dilution method; The presence of qacE/kl- sullgene, class 1, 2 and 3 integrase as well as gene cassettes embedded within class 1 integron were detected by polymerase chain reaction （PCR）. Results Ceftazidime and ticarcillin/clavulanic acid demonstrated a higher resistance rate of 63.8% and 75.9%; 31.3% of clinical Stenotrophomonas maltophilia isolates was resistant to trimethoprim/sulfamethoxazole; The gene of qacEA l-sull was detected in 18 of 83 isolates. And class 1 integrase was detected in 20 isolates. The identified gene cassettes within class 1 integron were acAd-catB8-aadA1, aac（6＇）- Ⅱ-blaCAB-8 and aar-3-dfrA27. And aar-3-dfrA27 was detected from SMA for the first time. The gene was submitted to GenBank database, and the accession number is KC748137. Conclusion The situation of resistance is fairly serious in this 83 clinical Stenotrophomonas maltophilia isolates, and the recommendations about antimicrobialtreatment must be updated regularly based on surveillance results. Maybe the rise of resistance rate to trimethoprim/ sulfamethoxazole relates to class 1 integron which also accelerated the spread of drug-resistant bacteria.