中文摘要：

目的研究山东地区汉族人群15个X—STR基因座的遗传多态性．建立法医学应用数据库。方法采用PrimerPremier5．0软件设计多重PCR引物，用4色荧光（FAM、VIC、NED、TET）进行标记，建立多重PCR体系，对山东地区无关汉族人群481例个体（女性295例，男性186例）的15个X染色体上筛选的STR基因座（DXSl0011、DXSl01、GATA165812、DXS6795、DXS6800、DXS6801、DXS6803、DXS7132、DXS7133、DXS7423、DXS7424、DXS8377、DXS8378、DXS9898和月脉TB）进行检测。结果所检测的15个X—STR基因座中，GATAl65812、DXS6800、DXS6803、DXS7133与DXS7423在中国山东汉族人群中具有中度多态性，其余10个基因座都具有高度多态性（PIC〉0．5，H〉0．5）。群体中男性样本之间没有检测到共享单倍型。结论构建的荧光标记复合扩增体系为建立中国山东汉族人群X—STR基因座群体遗传学数据库及其法医学应用提供了有效的手段。

英文摘要：

Objective To investigate the genetic polymorphisms of 15 X-STR loci in Shandong Han pop- ulation in order to establish the forensic application database. Methods The multi-PCR primers of these loci were designed by Primer Premier 5.0 software and labeled by 4 fluoresceins （FAM, VIC, NED and TET）. The developed multi-PCR was used to investigate 15 X-STR loci （DXSIO011, DXSIO1, GATA165B12, DXS6795, DXS6800, DXS6801, DXS6803, DXS7132, DXS7133, DXS7423, DXS7424, DXS8377, DXS8378, DXS9898 and HPRTB） selected from the X chromosome of 481 unrelated individuals （295 females and 186 males） in Shandong Han population. Results Among the 15 X-STR loci, GATA165B12, DXS6800, DXS6803, DXS7133 and DXS7423 showed moderate polymorphisms, while the rest 10 X-STR loci showed high polymorphisms （PIC〉0.5 and H〉0.5）. No shared haplotype was detected among the males in Shandong Han population. Conclusion The developed multi-PCR system with fluorescence detection provides an effective way to establish X-STR loci database of population genetics in Shandong Hart population and shows its forensic application.