中文摘要：

采用分子克隆技术,从拟南芥中分离到一个质膜H^＋-ATPase基因At AHA11和一个钙结合蛋白基因At CBL10。采用酶切连接的方法构建At AHA11-C-ter-p GADT7和At CBL10-p GBKT7酵母双杂交载体,用PEG/Li Ac法将At AHA11-C-ter-p GADT7转化到酵母菌Y187中,At CBL10-p GBKT7转化到酵母菌Y2HGold中。发现融合的二倍体酵母（At AHA11-C-ter-p GADT7×At CBL10-p GBKT7）可以在酵母选择性培养基SD-A-H-L-T＋X-α-Gal＋Ab A中生长。说明At CBL10蛋白可与AHA11蛋白的C末端互作,便于我们进一步研究它们的调节位点,为深入探讨CBL10调控细胞膜AHA11蛋白的功能提供一个技术平台。

英文摘要：

An H~＋-ATPase gene At AHA11 and a calcium-binding protein gene At CBL10 were isolated from Arabidopsis by molecular cloning technique. The yeast two hybrid expressing vectors At AHA11-C-ter-p GADT7 and At CBL10-p GBKT7 were constructed using enzyme digestion and ligation methods, and then transformed into the Y187 and Y2 HGold yeast strains by PEG/Li Ac methods, respectively. The diploid yeast fused by the transformed Y187 and Y2 HGold yeast strain could grow on the selective yeast plates（SD-A-H-L-T＋X-α-Gal＋Ab A）. The results illustrated that At CBL10 protein could interact with the C-terminal of AHA11 protein, which was convenient for studying the regulation sites of At CBL10 protein, and found a technical platform for researching the mechanism of CBL10 protein regulated plasma membrane AHA11 protein.