中文摘要：

目的研究蠕虫感染对过敏性哮喘的影响。方法将15只BALB/c小鼠随机分成3组,每组5只。Ⅰ组为血吸虫感染后OVA致敏组,Ⅱ组为单纯OVA致敏组,Ⅲ组为正常对照组。Ⅰ组小鼠首先建立日本血吸虫感染模型,于感染后第57~84d,与Ⅱ组小鼠一起以OVA(卵清白蛋白)诱导建立过敏性哮喘模型,第85d剖杀全部小鼠,收集脾脏、肺脏和支气管肺泡灌洗液。制备支气管肺泡灌洗液涂片以计数其中各类细胞;制备肺组织切片以检查肺部病理变化;培养脾细胞以检测培养上清中相关细胞因子水平。另外,还要检测支气管肺泡灌洗液中相关细胞因子水平。结果与Ⅱ组相比,Ⅰ组小鼠肺部嗜酸粒细胞数量显著减少(P<0.05),肺部炎症明显减轻(P<0.05),支气管肺泡灌洗液和脾细胞培养上清中IL-4和IL-5水平均降低(P<0.05),而IFN-γ水平无明显改变(P>0.05)。结论日本血吸虫感染对OVA诱导的过敏性哮喘有抑制作用。

英文摘要：

In order to study the influence of helminth infection on allergic asthma, 15 BALB/c mice were divided into three groups randomly. In group 1, mice were Schistosoma japonicum-infected and OVA-treated; in group 2, mice were treated with OVA only, and in group 3, mice without any treatment were taken as control. Firstly, the mice in group 1 were infected with Schistosoma japonicum; and then, from day 57 to day 84 after infection, mice in group 1 and group 2 were immunized and challenged with Ovalbumin(OVA) tog...