中文摘要：

采用N-酰化反应,将6-胺基β-环糊精（β-CD-6-E）固载到马来酸酐改性聚乳酸（MPLA）大分子链上,得到新型环糊精改性聚乳酸基生物材料（PLA-β-CD）。对材料的亲水性和细胞相容性进行了考察。静态水接触角和吸水率的测定结果表明,膜表面的静态水接触角由改性前的76.7°降低到改性后的72.1°,吸水率由18.3%提高到23.9%。采用倒置显微静观察成骨细胞在材料上的生长情况,结果表明,PLA-β-CD上细胞不仅形态上优于PLA,而且在数量上也有优势。MTT法测定了成骨细胞在材料上培养2、4、6、8d的增殖活力,结果表明,成骨细胞在PLA-β-CD的增殖活力明显大于PLA上的增殖活力。

英文摘要：

A novel cyclodextrin polymer（PLA-β-CD） was synthesized by N-acylation reaction of MPLA（maleic anhydride modified poly（d,l-lactic acid）） using hydrophilic mono（6-（2-aminoethyl）-amino-6-deoxy）-β-cyclodextrin（β-CD-6-E） as acylating agent.Surface wettability and cell biocompatibility of PLA-β-CD were investigated.The tests indicated that the static water contact angle of PLA-β-CD was decreased from 76.7to 72.1°comparing with PLA,while the water absorption ratio was increased from 18.3% to 23.9%.The morphology of osteoblasts on PLA-β-CD film was observed by invert light microscope and it revealed that the osteoblasts cultured on PLA-β-CD spread was wider than PLA while the cell density on the former was higher than the latter.Cell proliferation was determined by MTT assay at 2,4,6,8d,and the results showed that proliferation power of osteoblasts cultured on PLA-β-CD stronger than PLA.