中文摘要：

目的应用生物信息学分析软件预测细粒棘球蚴重组抗原P-29氨基酸序列的结构与功能。方珐应用NCBI、Expasy等在线生物信息学网站及DNAstar、Rasmol等软件包分析P-29与其他物种的同源序列，进行多序列同源比对；预测二级结构、三级结构；预测主要抗原表位等。结果细粒棘球绦虫重组抗原P-29与其他物种的同源性比对结果表明与多房棘球绦虫同源性最高为97％，依次为日本血吸虫73．9％，冈比亚按蚊70．5％，埃及伊蚊66．2％；预测该蛋白分子质量约为27ku，PI为5．69，具有1个跨膜区，位于212—215，8个抗原表位，其结构域位于5-238位。结论生物信息学预测结果提示该可能是免疫诊断、药物作用及疫苗潜在的靶分子。

英文摘要：

Objective To predict the structure and function of recombinant antigen P-29 of Echinococcus granulous （rEg. P-29） using bioinformatics method. Methods By online analysis at bioinformatics websites such as NCB[（http：//www. nibi. nlm. nih. gov/）and Expasy （http..//cn. expasy, org/）, and employing software packages such as DNAstar and Rasmolto do multi-sequence homological alignment, secondary structure and tertiary structure, antigenic epitope analysis, etc. Results Similarity of rEg. P-29 compared to Echinococcus multilocularis, Schistosoma japonicum, Anopheles Gambia and Aedes aegypti was 97%, 73.96%, 70.5%, 66.2% respectively. Analysis of the predicted protein indicated a molecular mass of 27 ku, PI was 5.69, one transmembrane region, function sites and eight antigen epitope were found. Conclusion rEg. P-29 might be a potential molecular target for immunodiagnosis, anti-hydatid drug and vaccine development.